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AB50795

Anti-ThrRS抗体[TARSF8H3]

Anti-ThrRS antibody [TARSF8H3]

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(1 Publication)

Mouse Monoclonal ThrRS antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TARS1.

查看别名

TARS, TARS1, Threonyl-tRNA synthetase, Threonyl-tRNA synthetase 1, ThrRS

2 Images
Immunocytochemistry - Anti-ThrRS antibody [TARSF8H3] (AB50795)
  • ICC

Unknown

Immunocytochemistry - Anti-ThrRS antibody [TARSF8H3] (AB50795)

ab50795, at a dilution of 1/5, staining ThrRS in HeLa cell by Immunocytochemistry. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.

Western blot - Anti-ThrRS antibody [TARSF8H3] (AB50795)
  • WB

Unknown

Western blot - Anti-ThrRS antibody [TARSF8H3] (AB50795)

All lanes:

Western blot - Anti-ThrRS antibody [TARSF8H3] (ab50795) at 1/50 dilution

All lanes:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 83 kDa

Observed band size: 80 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

TARSF8H3

亚型

IgG2a

不含载体蛋白

No

反应种属

Human

应用

WB, ICC

applications

免疫原

Recombinant Fragment Protein within Human TARS1. The exact immunogen used to generate this antibody is proprietary information.

P26639

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/5", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
纯化说明
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. It is sterile filtered through a 0.22 µm membrane.
存储溶液
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ThrRS also known as threonyl-tRNA synthetase is an enzyme that attaches the amino acid threonine to its corresponding tRNA (tRNA^Thr) an important step in the translation process during protein synthesis. ThrRS's molecular mass is approximately 80 kDa. It is expressed in various tissues across many organisms showing high conservation from bacteria to humans. This enzyme ensures accurate pairing of threonine with its tRNA which is critical for translating genetic information into functional proteins.
Biological function summary

Threonyl-tRNA synthetase plays a role in cellular function by ensuring proteins are synthesized accurately and efficiently. ThrRS is a standalone enzyme and does not form part of an enzyme complex. Its correct functioning is essential for maintaining cellular homeostasis and ensuring the fidelity of the genetic code. Incorrect aminoacylation by ThrRS can lead to translational errors which may manifest in malfunctioning proteins.

Pathways

The activity of threonyl-tRNA synthetase is integral to the protein synthesis pathway which is part of the larger biological process of gene expression. This pathway involves collaboration with other aminoacyl-tRNA synthetases and overlaps with translation mechanisms. ThrRS is related to proteins such as serine-tRNA synthetase and alanine-tRNA synthetase as they also participate in aminoacylation facilitating accurate protein synthesis by adding the right amino acids to tRNA molecules.

Problems associated with threonyl-tRNA synthetase can lead to conditions like Charcot-Marie-Tooth disease a disorder affecting peripheral nerves and certain types of cancer. Mutations in the ThrRS gene can alter its enzymatic activity or stability potentially disrupting the translation process. Moreover issues with ThrRS can interact with defective proteins like glycyl-tRNA synthetase further impacting cellular function and contributing to disease pathologies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction : threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr) (PubMed : 25824639, PubMed : 31374204). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage (By similarity).
See full target information TARS1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular endocrinology 584:112160 PubMed38266771

2024

PMM2 controls ERα levels and cell proliferation in ESR1 Y537S variant expressing breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Cipolletti,Filippo Acconcia
View all publications
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