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AB321913

重组Anti-Thrombospondin 2抗体[EPR26608-39] - BSA and Azide free (Detector)

Anti-Thrombospondin 2 antibody [EPR26608-39] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Thrombospondin 2 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.

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Tsp2, Thrombospondin-2

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Sandwich ELISA - Anti-Thrombospondin 2 antibody [EPR26608-39] - BSA and Azide free (Detector) (AB321913)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Thrombospondin 2 antibody [EPR26608-39] - BSA and Azide free (Detector) (AB321913)

Sandwich ELISA of ab321911 with the capture antibody ab321912 dilution at 2 ug/mL and detector antibody ab321913 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-Thrombospondin 2 antibody [EPR26608-39] - BSA and Azide free (Detector) (AB321913)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Thrombospondin 2 antibody [EPR26608-39] - BSA and Azide free (Detector) (AB321913)

Interpolated concentration of native Thrombospondin 2 was measured at different sample concentrations. Undiluted sample is as follows : mouse bone marrow extract (200 ug/mL). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Thrombospondin 2 antibody [EPR26608-39] - BSA and Azide free (Detector) (AB321913)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Thrombospondin 2 antibody [EPR26608-39] - BSA and Azide free (Detector) (AB321913)

Interpolated concentration of native Thrombospondin 2 was measured at different sample concentrations. Undiluted samples are as follows : mouse serum 0.625%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR26608-39

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody does not cross-react with mouse Thrombospondin 1.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

ab321913 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse/Rat Thrombospondin 2. The recommended pair for sandwich ELISA is:

Capture: ab321912, Mouse/Rat Thrombospondin 2 Capture Antibody (unconjugated)

Detector: ab321913, Mouse/Rat Thrombospondin 2 Detector Antibody (unconjugated)

The reference range value is 62.5-4000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: 100% PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Thrombospondin 2 also known as TSP2 is a glycoprotein with a molecular weight of approximately 150 kDa. It is an extracellular matrix protein found in a variety of tissues including vascular tissues and connective tissues. Expression occurs in fibroblasts endothelial cells and certain types of immune cells. TSP2 plays a role in cell-to-cell and cell-to-matrix interactions through its multi-domain structure allowing it to bind with a range of cellular receptors and matrix components.
Biological function summary

TSP2 modulates angiogenesis the process of new blood vessel formation. It functions as an inhibitor of angiogenesis contributing to the regulation of tissue repair and remodeling. TSP2 does not operate alone; it is part of a larger complex within the extracellular matrix that also involves other thrombospondins like Thrombospondin 1 with which it shares some functional similarities. These thrombospondins collaborate to influence cell adhesion and migration.

Pathways

TSP2 participates significantly in the TGF-β signaling pathway and the regulation of vascular endothelial growth factor (VEGF) activity. Through the TGF-β pathway TSP2 influences extracellular matrix remodeling and tissue fibrosis. Additionally its interaction with VEGF modulates angiogenesis where TSP2 acts as an antagonist counterbalancing the effects of VEGF. This interaction links TSP2 with regulatory mechanisms involving proteins like integrins which play roles in the cellular attachment to the matrix.

TSP2 connects notably with cancer and fibrosis. In cancer its angiogenesis-inhibiting properties can affect tumor growth and metastasis by limiting blood supply to the tumor a process that involves its interaction with matrix metalloproteinases. In fibrosis especially pulmonary and hepatic forms TSP2 influences tissue scarring and repair through its oppression of angiogenesis and interaction with the TGF-β pathway affecting fibrotic protein expression.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.
See full target information Thbs2
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