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Neuroscience Cell Type Marker Glia marker Astrocyte marker

Anti-Thrombospondin 1抗体[A6.1] (ab1823)

  • Datasheet
  • SDS
Reviews (19)Q&A (25)References (61)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
  • Flow Cytometry - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)

Key features and details

  • Mouse monoclonal [A6.1] to Thrombospondin 1
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Thrombospondin 1抗体[A6.1]
    参阅全部 Thrombospondin 1 一抗
  • 描述

    小鼠单克隆抗体[A6.1] to Thrombospondin 1
  • 宿主

    Mouse
  • 经测试应用

    适用于: Flow Cyt, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Rat, Human
  • 免疫原

    Full length protein corresponding to Human Thrombospondin 1. BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

  • 阳性对照

    • Tonsil
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 克隆

    单克隆
  • 克隆编号

    A6.1
  • 同种型

    IgG1
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Regulation
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Secreted
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic inhibitory factors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Thrombospondin 1 protein (ab52124)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1823于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF (4)
Use at an assay dependent concentration.
IHC-P (7)
Use at an assay dependent concentration.
说明
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.
  • 序列相似性

    Belongs to the thrombospondin family.
    Contains 2 EGF-like domains.
    Contains 1 laminin G-like domain.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 3 TSP type-1 domains.
    Contains 8 TSP type-3 repeats.
    Contains 1 VWFC domain.
  • 细胞定位

    Endoplasmic reticulum. Sarcoplasmic reticulum.
  • Target information above from: UniProt accession P07996 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7057 Human
    • Entrez Gene: 445442 Rat
    • Omim: 188060 Human
    • SwissProt: P07996 Human
    • Unigene: 164226 Human
    • Unigene: 732539 Human
    • 别名

      • Thbs1 antibody
      • Thrombospondin-1 antibody
      • TSP antibody
      • Tsp1 antibody
      • TSP1_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)

      Immunohistochemical analsysis of formalin-fixed paraffin-embedded human placenta, labeling Thrombospondin with ab1823.

    • Flow Cytometry - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
      Flow Cytometry - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
      Overlay histogram showing PC12 cells stained with ab1823 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1823, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
      Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)This image is courtesy of an anonymous Abreview
      ab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
      Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
      ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)

      ab1823 at a 1/25 dilution staining human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .

      This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.

      See Abreview

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (61)

    发表研究结果有使用 ab1823?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1823 被引用在 61 文献中.

    • Leng Y  et al. Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis. Cancer Cell Int 21:17 (2021). PubMed: 33407520
    • Chen S  et al. Exosomes derived from retinoblastoma cells enhance tumour deterioration by infiltrating the microenvironment. Oncol Rep 45:278-290 (2021). PubMed: 33416154
    • Qu S  et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
    • Semba S  et al. Synthetic PAMPS gel induces chondrogenic differentiation of ATDC5 cells via a novel protein reservoir function. J Biomed Mater Res A N/A:N/A (2020). PubMed: 32496623
    • Wang TY  et al. Maggot excretions/secretions promote diabetic wound angiogenesis via miR18a/19a - TSP-1 axis. Diabetes Res Clin Pract 165:108140 (2020). PubMed: 32277954
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 44 Abreviews or Q&A

    Western blot abreview for Anti-Thrombospondin antibody [A6.1]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Serum (Rabbit Aqueouse Humor, 20ul/well)
    Gel Running Conditions
    Reduced Denaturing (8-20%)
    Loading amount
    20 µg
    Specification
    Rabbit Aqueouse Humor, 20ul/well
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Xiaoqing Guo

    Verified customer

    提交于 Apr 30 2019

    Western blot abreview for Anti-Thrombospondin antibody [A6.1]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% mini gel)
    Sample
    Mouse Tissue lysate - whole (Penile Tissue)
    Specification
    Penile Tissue
    Blocking step
    SuperBlock Blocking Buffer (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Miss. Gwen Lagoda

    Verified customer

    提交于 Nov 11 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Thrombospondin antibody [A6.1]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Sample
    Pig Tissue sections (liver)
    Specification
    liver
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 29 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Sample
    Rabbit Tissue sections (splen rabbit)
    Specification
    splen rabbit
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 04 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 04 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Thrombospondin antibody [A6.1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MEFs)
    Specification
    MEFs
    Fixative
    Formaldehyde
    Permeabilization
    Yes - TBS Tw20
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 12 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Thrombospondin antibody [A6.1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (1BRG3 fibroblast)
    Specification
    1BRG3 fibroblast
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Tween 20
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 12 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Thrombospondin antibody [A6.1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Meriones unguiculatus Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Andrea Dannullis

    Verified customer

    提交于 Sep 25 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Thrombospondin antibody [A6.1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (intestinum)
    Specification
    intestinum
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    Yes - PBS/TritonX
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 18 2012

    Question

    Would just like to know if you have a positive control slide for mouse tissue that we might be able to use to help us validate our antibody. I am currently following the abcam IHC protocol for staining my slides.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 25 2013

    Answer


    There are a number of tissues that could serve as positive controls. We do not have sections of mouse tonsil, which is listed on the datasheet as a positive control, but sections of spleen (ab4613) or placenta (ab4612) are also good choices for IHC controls for this protein.

    Please note that ab1823 is a mouse antibody, so a secondary antibody anti-mouse IgG may stain endogenous IgG in the tissue section. To address this, please consider a mouse-on-mouse staining reagent such as ab127055.

    Read More

    Abcam Scientific Support

    回复于 Apr 25 2013

    1-10 of 44 Abreviews or Q&A

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