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Cardiovascular Blood Coagulation Regulatory

Anti-Thrombomodulin抗体[PBS-01] (ab6980)

  • Datasheet
  • SDS
Reviews (7)Q&A (9)References (20)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)
  • Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)

Key features and details

  • Mouse monoclonal [PBS-01] to Thrombomodulin
  • Suitable for: ELISA, WB, Flow Cyt, IHC-P
  • Reacts with: Rabbit, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Thrombomodulin抗体[PBS-01]
    参阅全部 Thrombomodulin 一抗
  • 描述

    小鼠单克隆抗体[PBS-01] to Thrombomodulin
  • 宿主

    Mouse
  • 经测试应用

    适用于: ELISA, WB, Flow Cyt, IHC-Pmore details
  • 种属反应性

    与反应: Rabbit, Human
    不与反应: Pig
  • 免疫原

    Recombinant full length protein corresponding to Human Thrombomodulin.
    Database link: P07204

  • 表位

    EGF domain 5 of thrombomodulin.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 6.50
    Constituents: 0.292% Sodium chloride, 4% Sucrose, 0.1% Tween, 0.41% Sodium acetate
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    PBS-01
  • 骨髓瘤

    Sp2
  • 同种型

    IgG1
  • 研究领域

    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Adhesion molecules ELISA kits
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human Thrombomodulin protein (ab98989)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6980于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
WB (3)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P (2)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • 组织特异性

    Endothelial cells are unique in synthesizing thrombomodulin.
  • 疾病相关

    Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • 序列相似性

    Contains 1 C-type lectin domain.
    Contains 6 EGF-like domains.
  • 翻译后修饰

    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P07204 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7056 Human
    • Omim: 188040 Human
    • SwissProt: P07204 Human
    • Unigene: 2030 Human
    • 别名

      • AHUS 6 antibody
      • AHUS6 antibody
      • BDCA 3 antibody
      • BDCA3 antibody
      • CD 141 antibody
      • CD141 antibody
      • CD141 antigen antibody
      • Fetomodulin antibody
      • Thbd antibody
      • THPH12 antibody
      • THRM antibody
      • Thrombomodulin antibody
      • TM antibody
      • TRBM_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)
      Ab6980 staining human normal lung endothelium. Staining is localized to the membrane.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)
      Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)
      Overlay histogram showing THP1 cells stained with ab6980 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (20)

    发表研究结果有使用 ab6980?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6980 被引用在 20 文献中.

    • Anand RP  et al. Design and testing of a humanized porcine donor for xenotransplantation. Nature 622:393-401 (2023). PubMed: 37821590
    • De León Rodríguez SG  et al. A Machine Learning Workflow of Multiplexed Immunofluorescence Images to Interrogate Activator and Tolerogenic Profiles of Conventional Type 1 Dendritic Cells Infiltrating Melanomas of Disease-Free and Metastatic Patients. J Oncol 2022:9775736 (2022). PubMed: 36276271
    • Okamoto H  et al. Recombinant Antithrombin Attenuates Acute Respiratory Distress Syndrome in Experimental Endotoxemia. Am J Pathol 191:1526-1536 (2021). PubMed: 34116023
    • Suzuki K  et al. Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia. Am J Pathol 189:1526-1535 (2019). PubMed: 31108101
    • Sobol N  et al. Synthesis and targeting of gold-coated 177Lu-containing lanthanide phosphate nanoparticles-A potential theranostic agent for pulmonary metastatic disease. APL Bioeng 2:016101 (2018). PubMed: 31069286
    View all Publications for this product

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Thrombomodulin antibody [PBS-01]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Aortic Endothelial Cells)
    Loading amount
    30 µg
    Specification
    Human Aortic Endothelial Cells
    Gel Running Conditions
    Reduced Denaturing (10% resolving gel, 5% stacking gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 13 2011

    Western blot abreview for Anti-Thrombomodulin antibody [PBS-01]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human cell line)
    Loading amount
    50 µg
    Specification
    Human cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 02 2008

    Western blot abreview for Anti-Thrombomodulin antibody [PBS-01]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HUVECs)
    Specification
    HUVECs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 % - PBST
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Thati Madhusudhan

    Verified customer

    提交于 Sep 21 2005

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