Anti-Thrombin抗体(ab48626)

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab48626. I would also appreciate if you can confirm some further details:

- Could you confirm when the product was ordered? This will help me trace the origin and internal lot details.
- Reviewing the Swiss-prot entry, I would expect a band at ˜65-70 kDa to be observed, however you state that you would expect a band of 30 kDa. Why would you expect a band size of 30 kDa? The antibody should detect thrombin and prothrombin.
- The antibody was generated against full length native protein so it is unlikely to recognize antihrombin.
- Is it possible to obtain an image so I can understand the problem in more detail?
- Was the sample prepared and resolved under reducing and denaturing conditions? The antibody has been characterized under such conditions.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Thank you for contacting me again. Detecting thrombin specifically is a very hard issue. As thrombin is part of pro thrombin and the proform undergoes activation during blood coagulation therefore it is difficult to differentiate both proteins. We have several products in our catalogue targeting thrombin, but they are all very likely to recognise pro thrombin as well. In order to obtain clearer thrombin bands, using protein degradation inhibitors are highly recommended (to avoid protein degradation products normally showing as lower target bands), as well as anti coagulants. Thrombin is a very special product, with different sites of glycosilation, producing bands at higher molecular weights. So in this particular case, sample preparation is crucial. However, I would be very happy to offer you a credit note for the price of this antibody, valid for a future purchase. I would really like to help you get the better results from the antibody, so please, let us know how you wish to proceed. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.