AB130996

Anti-THRAP5抗体

Name: THRAP5抗体 (ab130996)| Abcam中文官网
Brand: Abcam Limited
SKU: AB130996
Rating: 5 (1 reviews)

Anti-THRAP5 antibody

(1 Review)

(6 Publications)

Rabbit Polyclonal THRAP5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MED16 aa 550-600.

查看别名

DRIP92, THRAP5, MED16, Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Vitamin D3 receptor-interacting protein complex 92 kDa component, Trap95

2 Images

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Western blot - Anti-THRAP5 antibody (AB130996)

All lanes:

Western blot - Anti-THRAP5 antibody (ab130996) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 97 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-THRAP5 antibody (AB130996)

Detection of THRAP5 by Western Blot of Immunprecipitate.

ab130996 at 1µg/ml staining THRAP5 in HeLa whole cell lysate immunoprecipitated using ab130996 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Detection : Chemiluminescence with exposure times of 30 seconds

All lanes:

Immunoprecipitation - Anti-THRAP5 antibody (ab130996)

Predicted band size: 97 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human MED16 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2X0

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab130996 is affinity purified using an epitope specific to THRAP5 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

THRAP5 also known as TRIP13 and thyroid hormone receptor-associated protein 5 is a protein with a molecular mass of approximately 36 kDa. This protein usually operates in the nucleus as a part of transcriptional regulation processes. It is strongly expressed in various tissues including the liver kidney and brain indicating its widespread presence in the body. Mechanically THRAP5 interacts with nuclear hormone receptors and acts as a coactivator which means that it helps facilitate the transcription of specific genes.

Biological function summary

THRAP5 functions as part of a larger complex called the Mediator complex which is essential for the regulation of gene expression. This complex serves as a bridge linking transcriptional activators and the RNA polymerase II machinery thereby influencing the transcription of genes downstream. THRAP5 is involved in various regulatory roles that include assisting in the activation of nuclear receptors. Its involvement in the complex structure of Mediator indicates its role in controlling gene expression.

Pathways

THRAP5 involves itself in the regulation of transcriptional processes and may influence the ERK/MAPK signaling pathway. This pathway is important for cell growth proliferation and differentiation. The protein interacts with transcription factors and other proteins like ERK1/2 to modulate these pathways. Its role in facilitating communication between hormone receptors and transcriptional machinery positions it as an important regulatory hub in cellular signaling.

THRAP5 interacts with various proteins involved in cancer and metabolic disorders. Studies have linked its aberrant expression to certain cancers including breast cancer where it might play a role in tumor progression. Additionally metabolic conditions like obesity have shown connections to THRAP5 potentially through interactions with PPARs (peroxisome proliferator-activated receptors) which are important for metabolic regulation. Understanding the contribution of THRAP5 in these diseases could offer insights into novel therapeutic targets.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

See full target information MED16

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 41: PubMed34291801

2021

Function of cofactor Akirin2 in the regulation of gene expression in model human Caucasian neutrophil-like HL60 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Artigas-Jerónimo,Margarita Villar,Agustín Estrada-Peña,Adrián Velázquez-Campoy,Pilar Alberdi,José de la Fuente

Bioscience reports : PubMed34223621

2021

Function of cofactor Akirin2 in the regulation of gene expression in model human Caucasian neutrophil-like HL60 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Artigas-Jerónimo,Margarita Villar,Agustín Estrada-Peña,Adrián Velázquez-Campoy,Pilar Alberdi,José de la Fuente

The EMBO journal 40:e102509 PubMed34155658

2021

The SAGA complex regulates early steps in transcription via its deubiquitylase module subunit USP22.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy J Stanek,Victoria J Gennaro,Mason A Tracewell,Daniela Di Marcantonio,Kristen L Pauley,Sabrina Butt,Christopher McNair,Feng Wang,Andrew V Kossenkov,Karen E Knudsen,Tauseef Butt,Stephen M Sykes,Steven B McMahon

Vaccines 8: PubMed32046307

2020

A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Artigas-Jerónimo,Juan J Pastor Comín,Margarita Villar,Marinela Contreras,Pilar Alberdi,Israel León Viera,Leandro Soto,Raúl Cordero,James J Valdés,Alejandro Cabezas-Cruz,Agustín Estrada-Peña,José de la Fuente

Nature communications 7:11624 PubMed27211851

2016

Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription.

Applications

Species

Unspecified reactive species

Eri H Kobayashi,Takafumi Suzuki,Ryo Funayama,Takeshi Nagashima,Makiko Hayashi,Hiroki Sekine,Nobuyuki Tanaka,Takashi Moriguchi,Hozumi Motohashi,Keiko Nakayama,Masayuki Yamamoto

Molecular and cellular biology 36:407-20 PubMed26572828

2015

The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Sekine,Keito Okazaki,Nao Ota,Hiroki Shima,Yasutake Katoh,Norio Suzuki,Kazuhiko Igarashi,Mitsuhiro Ito,Hozumi Motohashi,Masayuki Yamamoto

View all publications

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Anti-THRAP5抗体

Anti-THRAP5 antibody

Western blot - Anti-THRAP5 antibody (AB130996)

Immunoprecipitation - Anti-THRAP5 antibody (AB130996)