重组Anti-THOC5抗体[EPR6905(B)] (ab124996)
![Western blot - Anti-THOC5 antibody [EPR6905(B)] (ab124996)](https://www.abcam.cn/ps/products/124/ab124996/Images/ab124996-139090-anti-thoc5-antibody-epr6905b-western-blot.jpg "Western blot - Anti-THOC5 antibody [EPR6905(B)] (ab124996)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6905(B)] to THOC5
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-THOC5抗体[EPR6905(B)]
参阅全部 THOC5 一抗 -
描述
兔单克隆抗体[EPR6905(B)] to THOC5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide within Human THOC5 aa 650-750. The exact sequence is proprietary.
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阳性对照
- 293T, HeLa, NIH 3T3 and A549 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 4.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6905(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124996于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Component the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and ALYREF/THOC4. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the THOC5 family. -
翻译后修饰
Phosphorylated on tyrosine upon binding to activated CSF1R; which causes a dissociation of the two proteins. Phosphorylation on Ser-5 and/or Ser-6 is required for nuclear export. Phosphorylated on Thr-328 in insulin-stimulated adipocytes (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8563 Human
- Entrez Gene: 107829 Mouse
- Entrez Gene: 360972 Rat
- Omim: 612733 Human
- SwissProt: Q13769 Human
- SwissProt: Q8BKT7 Mouse
- SwissProt: Q68FX7 Rat
- Unigene: 75361 Human
see all -
别名
- C22orf19 antibody
- Chromosome 22 open reading frame 19 antibody
- Fmip antibody
see all
图片
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Western blot - Anti-THOC5 antibody [EPR6905(B)] (ab124996)All lanes : Anti-THOC5 antibody [EPR6905(B)] (ab124996) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 79 kDa -
![OI-RD Scanning - Anti-THOC5 antibody [EPR6905(B)] (ab124996)](https://www.abcam.cn/ps/products/124/ab124996/Images/ab124996-139089-anti-thoc5-antibody-epr6905b.jpg)
OI-RD Scanning - Anti-THOC5 antibody [EPR6905(B)] (ab124996) -
![Anti-THOC5 antibody [EPR6905(B)] (ab124996)](https://www.abcam.cn/ps/products/124/ab124996/Images/ab124996-3-benefits-of-recombinant-antibodies.png)
Anti-THOC5 antibody [EPR6905(B)] (ab124996)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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