Anti-THOC2/Tho2抗体(ab129485)
Key features and details
- Rabbit polyclonal to THOC2/Tho2
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-THOC2/Tho2抗体 -
描述
兔多克隆抗体to THOC2/Tho2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human THOC2/Tho2 aa 1543-1593.
Database link: NP_001075019.1 -
阳性对照
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129485于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 183 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 183 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex.The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. -
序列相似性
Belongs to the THOC2 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 57187 Human
- Entrez Gene: 331401 Mouse
- Entrez Gene: 313308 Rat
- Omim: 300395 Human
- SwissProt: Q8NI27 Human
- SwissProt: B1AZI6 Mouse
- Unigene: 149991 Human
- Unigene: 259498 Mouse
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别名
- CXorf3 antibody
- DJ506G2.1 antibody
- hTREX120 antibody
see all
图片
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Western blot - Anti-THOC2/Tho2 antibody (ab129485)All lanes : Anti-THOC2/Tho2 antibody (ab129485) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 183 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THOC2/Tho2 antibody (ab129485)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling THOC2/Tho2 with ab129485 at 1/1000 (1µg/mg). Detection: DAB.
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Immunoprecipitation - Anti-THOC2/Tho2 antibody (ab129485)Lane 1: ab129485 at 1 µg/ml staining THOC2/Tho2 in HeLa whole cell lysate immunoprecipitated using ab129485 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Detection: Chemiluminescence
Exposure time: 1 second
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab129485 被引用在 3 文献中.
- Gu XJ et al. MiR-30e-3p inhibits gastric cancer development by negatively regulating THO complex 2 and PI3K/AKT/mTOR signaling. World J Gastrointest Oncol 14:2170-2182 (2022). PubMed: 36438699
- Barreiro-Alonso A et al. HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes. Cancers (Basel) 13:N/A (2021). PubMed: 34572914
- Hjorth-Jensen K et al. SPOP promotes transcriptional expression of DNA repair and replication factors to prevent replication stress and genomic instability. Nucleic Acids Res 46:9484-9495 (2018). PubMed: 30124983