重组Anti-Thioredoxin / TRX抗体[EPR6111] (ab109385)
![Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)](https://www.abcam.cn/ps/products/109/ab109385/Images/ab109385-468757-anti-thioredoxin-trx-antibody-epr6111-westernblot-thioredoxin-recombinant-protein-human.jpg "Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6111] to Thioredoxin / TRX
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Thioredoxin / TRX抗体[EPR6111]
参阅全部 Thioredoxin / TRX 一抗 -
描述
兔单克隆抗体[EPR6111] to Thioredoxin / TRX -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- MCF-7, THP-1, HeLa and HepG2 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.03 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6111 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109385于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 12 kDa.
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 12 kDa. |
靶标
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功能
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). -
序列相似性
Belongs to the thioredoxin family.
Contains 1 thioredoxin domain. -
翻译后修饰
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation. -
细胞定位
Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7295 Human
- Entrez Gene: 22166 Mouse
- Entrez Gene: 116484 Rat
- Omim: 187700 Human
- SwissProt: P10599 Human
- SwissProt: P10639 Mouse
- SwissProt: P11232 Rat
- Unigene: 435136 Human
see all -
别名
- ADF antibody
- ATL derived factor antibody
- ATL-derived factor antibody
see all
图片
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Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385) at 1/1000 dilution (Purified) + His-tagged human Thioredoxin recombinant protein (aa 1-105)
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa -
![Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)](https://www.abcam.cn/ps/products/109/ab109385/Images/ab109385-468756-anti-thioredoxin-trx-antibody-epr6111-westernblot-human-mouse-rat.jpg)
Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)All lanes : Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
Lane 5 : Mouse brain lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa -
![Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)](https://www.abcam.cn/ps/products/109/ab109385/Images/ab109385-4-benefits-of-recombinant-antibodies.png)
Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab109385 被引用在 6 文献中.
- Ding J et al. The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstruction. PLoS One 18:e0286903 (2023). PubMed: 37310976
- Huang J et al. Lyciumbarbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways. J Zhejiang Univ Sci B 24:157-171 (2023). PubMed: 36751701
- Zhang X et al. Inhibition of HSP90 S-nitrosylation alleviates cardiac fibrosis via TGFβ/SMAD3 signalling pathway. Br J Pharmacol 178:4608-4625 (2021). PubMed: 34265086
- Xie MZ et al. Glyoxal damages human aortic endothelial cells by perturbing the glutathione, mitochondrial membrane potential, and mitogen-activated protein kinase pathways. BMC Cardiovasc Disord 21:603 (2021). PubMed: 34922451
- Zhou R et al. Salvianolic acid A attenuated myocardial infarction-induced apoptosis and inflammation by activating Trx. Naunyn Schmiedebergs Arch Pharmacol 393:991-1002 (2020). PubMed: 31811327
- Bouitbir J et al. Imatinib and Dasatinib Provoke Mitochondrial Dysfunction Leading to Oxidative Stress in C2C12 Myotubes and Human RD Cells. Front Pharmacol 11:1106 (2020). PubMed: 32792947