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重组RabMAb

重组Anti-Thioredoxin / TRX抗体[EPR23843-48] - BSA and Azide free (ab273880)

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Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Flow Cytometry (Intracellular) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Flow Cytometry (Intracellular) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
  • Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23843-48] to Thioredoxin / TRX - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Thioredoxin / TRX抗体[EPR23843-48] - BSA and Azide free
    参阅全部 Thioredoxin / TRX 一抗

  • 描述

    兔单克隆抗体[EPR23843-48] to Thioredoxin / TRX - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
    不适用于: IP

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: NIH/3T3, Neuro 2a, C2C12 and PC-12 whole cell lysates; Mouse kidney, liver and testis tissue lysates; Rat kidney tissue lysate. IHC-P: Mouse testis and kidney tissue; Rat kidney tissue. ICC/IF: NIH/3T3 and C6 cells. Flow Cyt (intra): NIH/3T3 and C6 cells.

  • 常规说明

    ab273880 is the carrier-free version of ab270449.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273880于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 12 kDa).
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 12 kDa).
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

  • 序列相似性

    Belongs to the thioredoxin family.
    Contains 1 thioredoxin domain.

  • 翻译后修饰

    In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

  • 细胞定位

    Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ADF antibody
      • ATL derived factor antibody
      • ATL-derived factor antibody
      • DKFZp686B1993 antibody
      • MGC61975 antibody
      • SASP antibody
      • Surface associated sulphydryl protein antibody
      • Surface-associated sulphydryl protein antibody
      • testicular tissue protein Li 199 antibody
      • THIO_HUMAN antibody
      • Thioredoxin antibody
      • thioredoxin delta 3 antibody
      • TRDX antibody
      • TRX 1 antibody
      • Trx antibody
      • TRX1 antibody
      • TXN antibody
      • TXN delta 3 antibody
      • TXN protein antibody
      • zgc:92903 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Thioredoxin / TRX with ab273877 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Thioredoxin / TRX with abab273877 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat kidney. The section was incubated with ab273877 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).

    • Flow Cytometry (Intracellular) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Flow Cytometry (Intracellular) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C6 (Rat glial tumor glial cell) cells labelling Thioredoxin / TRX with ab273877 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877). 

       

       

    • Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Anti-Thioredoxin / TRX antibody [EPR23843-48] (ab273877) at 1/1000 dilution + Rat kidney tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 12 kDa
      Observed band size: 11 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).

    • Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      All lanes : Anti-Thioredoxin / TRX antibody [EPR23843-48] (ab273877) at 1/1000 dilution

      Lane 1 : Mouse kidney tissue lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : Mouse testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 12 kDa
      Observed band size: 11 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Thioredoxin / TRX with abab273877 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID: 20682242). The section was incubated with ab273877 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).

    • Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      All lanes : Anti-Thioredoxin / TRX antibody [EPR23843-48] (ab273877) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
      Lane 3 : C2C12 (mouse myoblasts myoblast), whole cell lysate
      Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 12 kDa
      Observed band size: 11 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: Lane 1, 2: 7.75 seconds Lane 3, 4: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).

    • Flow Cytometry (Intracellular) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Flow Cytometry (Intracellular) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Thioredoxin / TRX with ab273877 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 cells labelling Thioredoxin / TRX with ab273877 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and nuclear staining in C6 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Thioredoxin / TRX with abab273877 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on sertoli and leydig cells of mouse testis. The section was incubated with ab273877 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).

    • Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)
      Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273880？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273880 尚未被引用在任何文献中。

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