Anti-Thioredoxin / TRX抗体- C-terminal (ab226925)
Key features and details
- Rabbit polyclonal to Thioredoxin / TRX - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Thioredoxin / TRX抗体- C-terminal
参阅全部 Thioredoxin / TRX 一抗 -
描述
兔多克隆抗体to Thioredoxin / TRX - C-terminal -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Rhesus monkey
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免疫原
Synthetic peptide within Human Thioredoxin/ TRX (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P10599 -
阳性对照
- WB: H1299 and HeLa whole cell lysate. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226925于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
|
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| WB |
1/500 - 1/3000. Predicted molecular weight: 12 kDa.
|
| 说明 |
|---|
|
ICC/IF
1/100 - 1/1000. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 12 kDa. |
靶标
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功能
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). -
序列相似性
Belongs to the thioredoxin family.
Contains 1 thioredoxin domain. -
翻译后修饰
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation. -
细胞定位
Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7295 Human
- Omim: 187700 Human
- SwissProt: P10599 Human
- SwissProt: P29451 Rhesus monkey
- Unigene: 435136 Human
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别名
- ADF antibody
- ATL derived factor antibody
- ATL-derived factor antibody
see all
图片
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Western blot - Anti-Thioredoxin / TRX antibody - C-terminal (ab226925)All lanes : Anti-Thioredoxin / TRX antibody - C-terminal (ab226925) at 1/3000 dilution
Lane 1 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 12 kDa12% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody - C-terminal (ab226925)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Thioredoxin / TRX (green) using ab226925 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226925 尚未被引用在任何文献中。