重组Anti-Themis抗体[EPR7354] (ab129174)
![Western blot - Anti-Themis antibody [EPR7354] (ab129174)](https://www.abcam.cn/ps/products/129/ab129174/Images/Themis-Primary-antibodies-ab129174-1.jpg "Western blot - Anti-Themis antibody [EPR7354] (ab129174)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7354] to Themis
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Themis抗体[EPR7354]
参阅全部 Themis 一抗 -
描述
兔单克隆抗体[EPR7354] to Themis -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Themis aa 600-700. The exact sequence is proprietary.
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阳性对照
- Human thymus and Jurkat lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7354 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.
靶标
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功能
Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. -
序列相似性
Belongs to the themis family. -
翻译后修饰
Phosphorylated on Tyr residues quickly after TCR stimulation. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 387357 Human
- Omim: 613607 Human
- SwissProt: Q8N1K5 Human
- Unigene: 661756 Human
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别名
- C6orf190 antibody
- C6orf207 antibody
- MGC163388 antibody
see all
图片
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Western blot - Anti-Themis antibody [EPR7354] (ab129174)All lanes : Anti-Themis antibody [EPR7354] (ab129174) at 1/10000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa -
![Anti-Themis antibody [EPR7354] (ab129174)](https://www.abcam.cn/ps/products/129/ab129174/Images/ab129174-2-benefits-of-recombinant-antibodies.png)
Anti-Themis antibody [EPR7354] (ab129174)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab129174 被引用在 1 文献中.
- Davies JL et al. Increased THEMIS First Exon Usage in CD4+ T-Cells Is Associated with a Genotype that Is Protective against Multiple Sclerosis. PLoS One 11:e0158327 (2016). WB ; Human . PubMed: 27438997