Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
使用敲除细胞株进行验证重组RabMAb

重组Anti-TGN46抗体[EPR12676] (ab174280)

Submit a review Submit a question References (2)
Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
  • Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
  • Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280)
  • Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)
  • Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280)
  • Anti-TGN46 antibody [EPR12676] (ab174280)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12676] to TGN46
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TGN46抗体[EPR12676]
    参阅全部 TGN46 一抗

  • 描述

    兔单克隆抗体[EPR12676] to TGN46

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: A549, HepG2 and HeLa lysates (HeLa shows to be a low expressor). IHC-P: Human brain and kidney tissues. IP: Immunoprecipitation pellet from fetal liver lysate. ICC/IF: Hep G2 and A549 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR12676

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab174280于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.
说明
WB
1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    May be involved in regulating membrane traffic to and from trans-Golgi network.

  • 组织特异性

    Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.

  • 细胞定位

    Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
  • Information by UniProt

  • 数据库链接

    • 别名

      • TGN 46 antibody
      • TGN 51 antibody
      • TGN38 antibody
      • TGN38 homolog antibody
      • TGN38B antibody
      • TGN46 antibody
      • TGN48 antibody
      • TGN51 antibody
      • TGOLN2 antibody
      • TGON2_HUMAN antibody
      • Trans Golgi network integral membrane protein 2 antibody
      • Trans golgi network protein 2 antibody
      • Trans Golgi network protein TGN51 antibody
      • Trans-Golgi network integral membrane protein 2 antibody
      • Trans-Golgi network protein TGN51 antibody
      • TTGN2 antibody
      see all

    图片

    • Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
      Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
      All lanes : Anti-TGN46 antibody [EPR12676] (ab174280) at 1/1000 dilution

      Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 2 : TGOLN2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 51 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab174280 observed at 80 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

      ab174280 was shown to react with TGN46 in wild-type A549 cells in Western blot with loss of signal observed in TGOLN2 knockout cell line ab269510 (knockout cell lysate ab269672). Wild-type A549 and TGOLN2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab174280 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
      Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
      All lanes : Anti-TGN46 antibody [EPR12676] (ab174280) at 1/1000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 51 kDa
      Observed band size: 75-100 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST.

      Exposure: 10 seconds, 100 seconds.

    • Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280)
      Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280)
      ab174280 staining TGN46 in wild-type A549 cells (top panel) and TGOLN2 knockout A549 cells (bottom panel) (ab269510). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab174280 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
    • Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)
      Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)

      Immunoprecipitation. ab174280 at 1/1000 labeling TGN46 immunoprecipitated from Human fetal liver lysate using ab174280 at 1/10.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)

      Immunohistochemical analysis of paraffin embedded Human brain tissue labeling TGN46 with ab174280 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling TGN46 with ab174280 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280)
      Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280)

      ab174280 staining TGN46 in HepG2 cells (top panel - positive control) and HeLa cells (bottom panel - negative control, low TGN46 expression). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab174280 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

    • Anti-TGN46 antibody [EPR12676] (ab174280)
      Anti-TGN46 antibody [EPR12676] (ab174280)

    文献 (2)

    发表研究结果有使用 ab174280？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab174280 被引用在 2 文献中.

    • Smith TJ  et al. Interplay between Furin and Sialoglycans in Modulating Adeno-Associated Viral Cell Entry. J Virol 97:e0009323 (2023). PubMed: 37097176
    • Han J  et al. HSP90 inhibition targets autophagy and induces a CASP9-dependent resistance mechanism in NSCLC. Autophagy 14:958-971 (2018). PubMed: 29561705

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab174280.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com