重组Anti-TGIF抗体[EP637Y] (ab52955)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP637Y] to TGIF
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TGIF抗体[EP637Y]
参阅全部 TGIF 一抗 -
描述
兔单克隆抗体[EP637Y] to TGIF -
宿主
Rabbit -
特异性
ab52955 detects both phosphorylated and unphosphorylated TGIF. -
经测试应用
适用于: WB, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa and K562 cell lysate. IP: HeLa whole cell lysate (ab150035)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP637Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52955于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 45 kDa).
For unpurified use at 1/20000 |
|
IP |
1/100.
For unpurified use at 1/30 |
说明 |
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WB
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 45 kDa). For unpurified use at 1/20000 |
IP
1/100. For unpurified use at 1/30 |
靶标
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功能
Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities. -
疾病相关
Holoprosencephaly 4 -
序列相似性
Belongs to the TALE/TGIF homeobox family.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7050 Human
- Omim: 602630 Human
- SwissProt: Q15583 Human
- Unigene: 373550 Human
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别名
- 5' TG 3' interacting factor antibody
- 5''-TG-3''-interacting factor 1 antibody
- Homeobox protein TGIF antibody
see all
图片
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ab52955 (purified) at 1:100 dilution (2µg) immunoprecipitating TGIF in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab52955 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52955 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Anti-TGIF antibody [EP637Y] (ab52955) at 0.4 µg/ml (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Anti-TGIF antibody [EP637Y] (ab52955) at 1/1000 dilution (Unpurifed) + K562 cell lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab52955 被引用在 7 文献中.
- Ju Q et al. CtBP2 interacts with TGIF to promote the progression of esophageal squamous cell cancer through the Wnt/β‑catenin pathway. Oncol Rep 47:N/A (2022). PubMed: 34878149
- Zhang J et al. TGIF1 Knockdown Inhibits the Proliferation and Invasion of Gastric Cancer via AKT Signaling Pathway. Cancer Manag Res 13:2603-2612 (2021). PubMed: 33776478
- Haider MT et al. Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment. Breast Cancer Res 22:34 (2020). PubMed: 32272947
- Zhang T et al. Aberrant super-enhancer landscape reveals core transcriptional regulatory circuitry in lung adenocarcinoma. Oncogenesis 9:92 (2020). PubMed: 33070167
- Liu X et al. The Chinese Medicine, Shezhi Huangling Decoction, Inhibits the Growth and Metastasis of Glioma Cells via the Regulation of miR-1298-5p/TGIF1 Axis. Cancer Manag Res 12:5677-5687 (2020). PubMed: 32765071
- Saito H et al. TG-interacting factor 1 (Tgif1)-deficiency attenuates bone remodeling and blunts the anabolic response to parathyroid hormone. Nat Commun 10:1354 (2019). PubMed: 30902975
- Yen-Ping Ho J et al. Transforming growth interacting factor expression in leiomyoma compared with myometrium. Fertil Steril 94:1078-1083 (2010). WB . PubMed: 19524896