重组Anti-TGFBI抗体[EPR12078(B)]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (AB170874)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TGFBI using ab170874 at a 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (AB170874)

Immunofluorescent staining of Y79 cells labeling TGFBI using ab170874 at a 1/50 dilution (green) and DAPI nuclear staining (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (AB170874)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TGFBI using ab170874 at a 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-TGFBI antibody [EPR12078(B)] (AB170874)

All lanes:

Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 75 kDa

false

• WB

CiteAb

Western blot - Anti-TGFBI antibody [EPR12078(B)] (AB170874)

Western Blotting using Anti-TGFBI antibody [EPR12078(B)], ab170874. Publication image from Liu, Y. et al., 2019, Nat Commun, 30967542. Legend direct from paper.

TGFBI is a functional ceRNA for miR-21 to regulate EMT. a Graph showing the number of miR-21 MREs extrapolated from RNA-seq data during TGF-β-induced EMT in A549 cells using targetScan-based predictions. b Graph showing the dynamics of modeled 8-mer miR-21 binding site occupancy during TGF-β-induced EMT in A549 cells using targetScan-based predictions. c Graph showing the level of indicated RNAs in A549 cells during TGF-β-induced EMT subjected to the indicated treatments. d Same as (c) for the immunoblotting analysis. e A549 cells undergoing TGF-β-induced EMT were treated as indicated and subjected to a migration assay (left panel) and an invasion assay (right panel). Scale bars : 100 µm. The migrated or invaded cells were quantified (bar charts). n = 6. f Graph showing the level of indicated RNAs in A549 cells during TGF-β-induced EMT subjected to the indicated treatments, using cells whose DICER1 has been knocked out with CRISPR-Cas9. g Same as (f) for the immunoblotting analysis. h Kaplan–Meier survival analyses based on the overexpression of TGFBI in three independent lung cancer data sets. n = 3; error bars indicate the means ± s.d. *p < 0.01, determined using a two-tailed Student’s t test. Source data are provided as a Source Data file

false

• WB

CiteAb

Western blot - Anti-TGFBI antibody [EPR12078(B)] (AB170874)

Western Blotting using Anti-TGFBI antibody [EPR12078(B)], ab170874. Publication image from Liu, Y. et al., 2019, Nat Commun, 30967542. Legend direct from paper.

TGFBI is a functional ceRNA for miR-21 to regulate EMT. a Graph showing the number of miR-21 MREs extrapolated from RNA-seq data during TGF-β-induced EMT in A549 cells using targetScan-based predictions. b Graph showing the dynamics of modeled 8-mer miR-21 binding site occupancy during TGF-β-induced EMT in A549 cells using targetScan-based predictions. c Graph showing the level of indicated RNAs in A549 cells during TGF-β-induced EMT subjected to the indicated treatments. d Same as (c) for the immunoblotting analysis. e A549 cells undergoing TGF-β-induced EMT were treated as indicated and subjected to a migration assay (left panel) and an invasion assay (right panel). Scale bars : 100 µm. The migrated or invaded cells were quantified (bar charts). n = 6. f Graph showing the level of indicated RNAs in A549 cells during TGF-β-induced EMT subjected to the indicated treatments, using cells whose DICER1 has been knocked out with CRISPR-Cas9. g Same as (f) for the immunoblotting analysis. h Kaplan–Meier survival analyses based on the overexpression of TGFBI in three independent lung cancer data sets. n = 3; error bars indicate the means ± s.d. *p < 0.01, determined using a two-tailed Student’s t test. Source data are provided as a Source Data file

false