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Anti-TGF beta 3抗体(ab53727)

Submit a review Q&A (2)References (1)
Western blot - Anti-TGF beta 3 antibody (ab53727)

    Key features and details

    • Rabbit polyclonal to TGF beta 3
    • Suitable for: WB
    • Reacts with: African green monkey
    • Isotype: IgG

    概述

    • 产品名称

      Anti-TGF beta 3抗体
      参阅全部 TGF beta 3 一抗

    • 描述

      兔多克隆抗体to TGF beta 3

    • 宿主

      Rabbit

    • 特异性

      Detects endogenous levels of total TGF ß3 protein.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: African green monkey
      预测可用于: Mouse, Rat

    • 免疫原

      Synthetic peptide derived from human TGF ß3.

    • 阳性对照

      • Extracts from COS7 cells.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab53727于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/500 - 1/1000. Predicted molecular weight: 47 kDa.
    说明
    WB
    1/500 - 1/1000. Predicted molecular weight: 47 kDa.

    靶标

    • 功能

      Involved in embryogenesis and cell differentiation.

    • 疾病相关

      Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.

    • 序列相似性

      Belongs to the TGF-beta family.

    • 细胞定位

      Secreted.
    • Information by UniProt

    • 数据库链接

      • 别名

        • ARVD antibody
        • ARVD1 antibody
        • FLJ16571 antibody
        • LDS5 antibody
        • MGC105479 antibody
        • MGC118722 antibody
        • prepro-transforming growth factor beta-3 antibody
        • RNHF antibody
        • TGF beta 3 antibody
        • TGF beta3 antibody
        • TGF-beta-3 antibody
        • TGFB 3 antibody
        • Tgfb3 antibody
        • TGFB3_HUMAN antibody
        • transforming growth factor beta 3 antibody
        • Transforming growth factor beta-3 antibody
        see all

      图片

      • Western blot - Anti-TGF beta 3 antibody (ab53727)
        Western blot - Anti-TGF beta 3 antibody (ab53727)
        All lanes : Anti-TGF beta 3 antibody (ab53727) at 1/500 dilution

        Lane 1 : extracts from COS7 cells
        Lane 2 : extracts from COS7 cells with immunizing peptide

        Performed under reducing conditions.

        Predicted band size: 47 kDa
        Observed band size: 11 kDa why is the actual band size different from the predicted?
        Additional bands at: 65 kDa (possible non-specific binding)



        The active molecule is a nonglycosylated, homodimer comprised of 13-kDa polypeptide chains linked by disulphide bonds.

      文献 (1)

      发表研究结果有使用 ab53727？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab53727 被引用在 1 文献中.

      • Ko T  et al. Cardiac fibroblasts regulate the development of heart failure via Htra3-TGF-β-IGFBP7 axis. Nat Commun 13:3275 (2022). PubMed: 35672400

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      Dear receiver,

      We bought antibodies against TGFB2 (ab36495) and TGFB3 (ab53727) to use in WB and we are a bit confused by the only predicted band size given in your datasheet; 50 kDa for TGFB2 and 47 kDa for TGFB3.

      1.Are these sizes for the full size pre-pro-TGFB versions?

      2.Should one not also detect the C-term mature proteins of around 12-13 kDa as well as possible dimers (approx. 25 kDa)?

      3.Do these proteins maybe form tetramers of approx 47-50 kDa?

      In the spec sheet for TGFB1 (ab9758) the only predicted bands are the monomer (12 kDa) and the dimer (25 kDa) so the band size info for TGFB2 and -3 was not in line with that and therefore a bit confusing.

      Thanks,

      Read More
      Answer

      Thank you for your enquiry.

      ab9758 - the major band at44.3 kDa corresponding to the unprocessed (pre-pro-TGF beta 1) form, the mature processed TGF beta 1 is a 25 kD homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. As the Western blot image indicates a 25 kDa band is seen in the non-reducing lane and a 12 kDa band in the reducing lane. Please visit UniProt website for further information at: http://www.uniprot.org/uniprot/P01137

      ab36495 - the major band at 47.8 kDa corresponding to the unprocessed form;the mature (processed)can form homodimers. UniProt: http://www.uniprot.org/uniprot/P61812

      ab53727 - the major band at 47.3 kDacorresponding to the unprocessed form, the mature (processed) can form homodimers. UniProt:http://www.uniprot.org/uniprot/P10600

      I hope this helps and if I can assist further, please do not hesitate to contact me.

      Read More

      Question

      Can you provide me with information on your antibody ab53727 (anti human tgf b3), what is the peptide length of the immunogen? Does the antibody recognize other members of the tgf family? Thank you in advance.

      Read More
      Answer

      The immunogen is 10aa long. The cross-reactivity against TGFb1 and TGFb2 has not been determined. However, the homology with the immunogen is 80% for both sequences. Thus, cross-reactivity could be possible.

      Read More

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