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重组RabMAb

重组Anti-TGF beta 1 + 2抗体[EPR5443] (ab124894)

Submit a review Q&A (1)References (1)
Western blot - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
  • Western blot - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
  • Western blot - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
  • OI-RD Scanning - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
  • Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5443] to TGF beta 1 + 2
  • Suitable for: WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TGF beta 1 + 2抗体[EPR5443]
    参阅全部 TGF beta 1 + 2 一抗

  • 描述

    兔单克隆抗体[EPR5443] to TGF beta 1 + 2

  • 宿主

    Rabbit

  • 特异性

    ab124894 recognises TGF beta 1 and 2.

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Human
    不与反应: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • A549 lysate, K562 lysate and L929 lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124894于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 44, 48 kDa.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 44, 48 kDa.
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

靶标

  • 相关性

    Function: TGF beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. The inactive form consists of a TGFB1 homodimer non-covalently linked to a latency-associated peptide (LAP) homodimer. The inactive complex can contain a latent TGFB1-binding protein. The active form is a homodimer of mature TGFB1; disulfide-linked. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. Interacts with ASPN. Function: TGF beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Tissue specificity: TGF beta 1 is highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Similarity: Both belong to the TGF-beta family.

  • 细胞定位

    Secreted

  • 数据库链接

    see all

  • 别名

    • TGF-beta-1 antibody
    • TGF-beta-2 antibody
    • Transforming growth factor Beta 1 antibody
    • Transforming growth factor Beta 2 antibody

图片

  • Western blot - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
    Western blot - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
    All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : TGFB2 knockout A549 cell lysate
    Lane 3 : U-2 OS cell lysate
    Lane 4 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 44, 48 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Western blot: Anti-TGFB2 antibody [EPR5443] (ab124894) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab124894 was shown to bind specifically to TGFB2. A band was observed at 48 kDa in wild-type A549 cell lysates with no signal observed at this size in TGFB2 knockout cell line. To generate this image, wild-type and TGFB2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

  • Western blot - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
    Western blot - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
    All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : K562 lysate
    Lane 3 : L929 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44, 48 kDa

  • Western blot - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
    Western blot - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
    All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution

    Lane 1 : TGF beta 2 recombinant protein
    Lane 2 : TGF beta 1 recombinant protein

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44, 48 kDa

  • OI-RD Scanning - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
    OI-RD Scanning - Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
    Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)

文献 (1)

发表研究结果有使用 ab124894？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab124894 被引用在 1 文献中.

  • Huang PS  et al. The Long Non-Coding RNA MIR503HG Enhances Proliferation of Human ALK-Negative Anaplastic Large-Cell Lymphoma. Int J Mol Sci 19:N/A (2018). WB . PubMed: 29758012

客户评价及客户问答

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Question

Why is the antibody listed as not reacting with mouse and rat? Data?
homology is 100%

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Answer


The lab let me know the following regarding their testing in mouse and rat:
Even though there is 100% sequence homology with the mouse and rat proteins, we still typically test for cross reactivity before recommending them for use with other species. In this case, ab124894 did not produce a band at the predicted MW in WB with mouse and rat samples (brain, heart kidney, lung and spleen as well as C6, Raw264.7, PC-12, NIH/3T3 and L929 cell lines).

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