Anti-TFIIF抗体(ab28179)
Key features and details
- Rabbit polyclonal to TFIIF
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TFIIF抗体
参阅全部 TFIIF 一抗 -
描述
兔多克隆抗体to TFIIF -
宿主
Rabbit -
特异性
This antibody reacts with the RAP74 subunit of TFIIF. -
经测试应用
适用于: ChIPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to TFIIF.
Database link: P35269 -
阳性对照
- HeLa cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.9
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28179于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP | (1) |
Use 5-10 µg for 25 µg of chromatin.
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| 说明 |
|---|
|
ChIP
Use 5-10 µg for 25 µg of chromatin. |
靶标
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相关性
The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 2962 Human
- Entrez Gene: 2963 Human
- Omim: 189968 Human
- Omim: 189969 Human
- SwissProt: P13984 Human
- SwissProt: P35269 Human
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别名
- ATP dependent helicase GTF2F2 antibody
- BTF 4 antibody
- BTF4 antibody
see all
图片
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ChIP - Anti-TFIIF antibody (ab28179)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28179 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (5)
ab28179 被引用在 5 文献中.
- Stanek TJ et al. The SAGA complex regulates early steps in transcription via its deubiquitylase module subunit USP22. EMBO J 40:e102509 (2021). PubMed: 34155658
- Narain A et al. Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination. Mol Cell 81:3110-3127.e14 (2021). PubMed: 34233157
- Kalkat M et al. MYC Protein Interactome Profiling Reveals Functionally Distinct Regions that Cooperate to Drive Tumorigenesis. Mol Cell 72:836-848.e7 (2018). PubMed: 30415952
- Hogel M et al. Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression. PLoS One 7:e41152 (2012). PubMed: 22815947
- Diamant G et al. DSIF restricts NF-?B signaling by coordinating elongation with mRNA processing of negative feedback genes. Cell Rep 2:722-31 (2012). WB, ChIP ; Human . PubMed: 23041311
