AB28179

Anti-TFIIF抗体

Name: TFIIF抗体 (ab28179)| Abcam中文官网
Brand: Abcam Limited
SKU: AB28179
Price: 4606 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-TFIIF antibody

(2 Reviews)

(6 Publications)

Rabbit Polyclonal GTF2F1 antibody. Suitable for ChIP and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GTF2F1.

查看别名

RAP74, GTF2F1, General transcription factor IIF subunit 1, General transcription factor IIF 74 kDa subunit, Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74, TFIIF-alpha

1 Images

ChIP

Unknown

ChIP - Anti-TFIIF antibody (AB28179)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25μg of chromatin, 8μg of ab28179 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ChIP

applications

免疫原

Recombinant Full Length Protein corresponding to Human GTF2F1.

P35269

特异性

This antibody reacts with the RAP74 subunit of TFIIF.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "5-10 µg for 25 µg chromatin", "ChIP-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.9 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

See full target information GTF2F1

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Apoptosis : an international journal on programmed cell death 27:762-777 PubMed35779185

2022

Hypoxic condition induced H3K27me3 modification of the LncRNA Tmem235 promoter thus supporting apoptosis of BMSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Zhang,Hong Luo,Wuxun Peng,Lei Wang,Tao Wang,Zhihong Xie,Jian Zhang,Wentao Dong,Xiaohan Zheng,Gang Liu,Xuesong Zhu,Qinglin Kang,Xiaobin Tian

Molecular cell 81:3110-3127.e14 PubMed34233157

2021

Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination.

Applications

Unspecified application

Species

Unspecified reactive species

Ashwin Narain,Pranjali Bhandare,Bikash Adhikari,Simone Backes,Martin Eilers,Lars Dölken,Andreas Schlosser,Florian Erhard,Apoorva Baluapuri,Elmar Wolf

The EMBO journal 40:e102509 PubMed34155658

2021

The SAGA complex regulates early steps in transcription via its deubiquitylase module subunit USP22.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy J Stanek,Victoria J Gennaro,Mason A Tracewell,Daniela Di Marcantonio,Kristen L Pauley,Sabrina Butt,Christopher McNair,Feng Wang,Andrew V Kossenkov,Karen E Knudsen,Tauseef Butt,Stephen M Sykes,Steven B McMahon

Molecular cell 72:836-848.e7 PubMed30415952

2018

MYC Protein Interactome Profiling Reveals Functionally Distinct Regions that Cooperate to Drive Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Manpreet Kalkat,Diana Resetca,Corey Lourenco,Pak-Kei Chan,Yong Wei,Yu-Jia Shiah,Natasha Vitkin,Yufeng Tong,Maria Sunnerhagen,Susan J Done,Paul C Boutros,Brian Raught,Linda Z Penn

Cell reports 2:722-31 PubMed23041311

2012

DSIF restricts NF-κB signaling by coordinating elongation with mRNA processing of negative feedback genes.

Applications

ChIP, WB

Species

Human, Human

Gil Diamant,Liat Amir-Zilberstein,Yuki Yamaguchi,Hiroshi Handa,Rivka Dikstein

PloS one 7:e41152 PubMed22815947

2012

Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Hogel,Robert B Laprairie,Eileen M Denovan-Wright

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TFIIF抗体

Anti-TFIIF antibody

ChIP - Anti-TFIIF antibody (AB28179)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性