Anti-TFII I (phospho Y248)抗体(ab63344)
Key features and details
- Rabbit polyclonal to TFII I (phospho Y248)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TFII I (phospho Y248)抗体
参阅全部 TFII I 一抗 -
描述
兔多克隆抗体to TFII I (phospho Y248) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human TFII I aa 200-300 (phospho Y248).
Database link: P78347 -
阳性对照
- Extracts from LOVO cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63344于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). |
靶标
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功能
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. -
组织特异性
Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues. -
疾病相关
Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
序列相似性
Belongs to the TFII-I family.
Contains 6 GTF2I-like repeats. -
翻译后修饰
Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
Sumoylated. -
细胞定位
Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2969 Human
- Entrez Gene: 14886 Mouse
- Entrez Gene: 353256 Rat
- Omim: 601679 Human
- SwissProt: P78347 Human
- SwissProt: Q9ESZ8 Mouse
- SwissProt: Q5U2Y1 Rat
- Unigene: 647041 Human
see all -
别名
- BAP 135 antibody
- BAP-135 antibody
- BAP135 antibody
see all
图片
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All lanes : Anti-TFII I (phospho Y248) antibody (ab63344) at 1/500 dilution
Lane 1 : extracts from LOVO cells, without immunising peptide
Lane 2 : extracts from LOVO cells, with immunising peptide
Predicted band size: 112 kDa
Observed band size: 112 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
The amount of cell extracts loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab63344 被引用在 1 文献中.
- Gui F et al. A non-covalent inhibitor XMU-MP-3 overrides ibrutinib-resistant BtkC481S mutation in B-cell malignancies. Br J Pharmacol 176:4491-4509 (2019). PubMed: 31364164