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Tags & Cell Markers Cell Type Markers Tumor Associated

Anti-TFEB抗体(ab2636)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (47)

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Western blot - Anti-TFEB antibody (ab2636)
  • Western blot - Anti-TFEB antibody (ab2636)

Key features and details

  • Goat polyclonal to TFEB
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-TFEB antibody [EPR22940-151] (ab270604)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-TFEB抗体
    参阅全部 TFEB 一抗
  • 描述

    山羊多克隆抗体to TFEB
  • 宿主

    Goat
  • 特异性

    This antibody is raised against TCFEB. However a customer reports that this antibody cross reacts with mouse TFE3 and MITF (other members of transcription factor family Mi/Tfe) in over-expressing cell lines.
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Human TFEB aa 2-14 (N terminal).
    Sequence:

    ASRIGLRMQLMRE


    Database link: P19484
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • 购买相配的WB阳性对照Recombinant Human TFEB protein
  • 常规说明

    Based on sequence homology, TFEB isoforms 1 and 2 (NP_009093.1 and NP_001161299.2) with MW of 52.9 and 54.2 kDa respectively can be detected by this antibody

    Principal Names - TFEB; transcription factor EB; TCFEB; T-cell transcription factor EB Official Gene Symbol - TFEB GenBank Accession Number – NP_009093 LocusLink ID - 7942 (human); 21425 (mouse)  

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • BALB/3T3 whole cell lysate (ab7901)
  • Recombinant Protein

    • Recombinant Human TFEB protein (ab152831)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2636于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
Use a concentration of 0.3 - 1 µg/ml.

Primary incubation 1 hour at room temperature. Blocking in milk. 

Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively. 

 

说明
WB
Use a concentration of 0.3 - 1 µg/ml.

Primary incubation 1 hour at room temperature. Blocking in milk. 

Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively. 

 

靶标

  • 功能

    Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.
  • 序列相似性

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 结构域

    The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region.
  • 翻译后修饰

    Sumoylated; does not affect dimerization with MITF.
  • 细胞定位

    Cytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.
  • Target information above from: UniProt accession P19484 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 510032 Cow
    • Entrez Gene: 609046 Dog
    • Entrez Gene: 7942 Human
    • Entrez Gene: 21425 Mouse
    • Entrez Gene: 100157940 Pig
    • Entrez Gene: 316214 Rat
    • Omim: 600744 Human
    • SwissProt: P19484 Human
    • SwissProt: Q9R210 Mouse
    • Unigene: 485360 Human
    • Unigene: 715310 Human
    • Unigene: 2305 Mouse
    see all
  • 别名

    • Alpha TFEB antibody
    • AlphaTFEB antibody
    • bHLHe35 antibody
    • Class E basic helix-loop-helix protein 35 antibody
    • T cell transcription factor EB antibody
    • TCFEB antibody
    • TFEB antibody
    • TFEB_HUMAN antibody
    • Transcription factor EB antibody
    see all

图片

  • Western blot - Anti-TFEB antibody (ab2636)
    Western blot - Anti-TFEB antibody (ab2636)
    Lane 1 : Anti-TFEB antibody (ab2636) at 0.5 µg/ml
    Lane 2 : Anti-TFEB antibody (ab2636) at 0.3 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate


    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Western blot - Anti-TFEB antibody (ab2636)
    Western blot - Anti-TFEB antibody (ab2636)
    Anti-TFEB antibody (ab2636) at 1 µg/ml + Human thymus lysate in RIPA buffer at 35 µg

    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (47)

发表研究结果有使用 ab2636?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2636 被引用在 47 文献中.

  • Jiang Y  et al. Basic fibroblast growth factor accelerates myelin debris clearance through activating autophagy to facilitate early peripheral nerve regeneration. J Cell Mol Med 25:2596-2608 (2021). PubMed: 33512767
  • Tan A  et al. TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy-lysosomal biogenesis. Cell Death Dis 12:343 (2021). PubMed: 33795648
  • Li R  et al. RIP3 impedes transcription factor EB to suppress autophagic degradation in septic acute kidney injury. Cell Death Dis 12:593 (2021). PubMed: 34103472
  • Rao S  et al. Salmonella and S. aureus Escape From the Clearance of Macrophages via Controlling TFEB. Front Microbiol 11:573844 (2020). PubMed: 33324360
  • Mingione A  et al. Inhibition of Sphingolipid Synthesis as a Phenotype-Modifying Therapy in Cystic Fibrosis. Cell Physiol Biochem 54:110-125 (2020). PubMed: 31999897
View all Publications for this product

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-TFEB antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEFs)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 04 2018

Western blot abreview for Anti-TFEB antibody - ChIP Grade

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 10% gel)
Loading amount
30 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Insu Kwon

Verified customer

提交于 Dec 10 2015

Western blot abreview for Anti-TFEB antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
35 µg
Specification
Brain
Blocking step
Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 22 2015

Western blot abreview for Anti-TFEB antibody - ChIP Grade

Average
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (breast cancer cell lines)
Loading amount
40 µg
Specification
breast cancer cell lines
Gel Running Conditions
Reduced Denaturing (8 % acrylamide gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 01 2010

Question

Hello, I recently purchased Anti-TFEB antibody - ChIP Grade (ab2636). Since it says that it is "ChIP grade,' my lab is planning on doing a few experiments involving ChIP, but before we do so we were wondering what genes/promotors abcam has used to verify that this antibody is suitable for ChIP? As much detailed information including controls, cell/tissue types probed, reaction conditions and buffers, etc. would be extremely helpful.

Read More

Abcam community

Verified customer

Asked on May 10 2012

Answer

Thank you for your inquiry.

We do not batch test this antibody for ChIP in-house, but this antibody has been successfully used in ChIP as described in the following papers:

Esumi N et al. VMD2 promoter requires two proximal E-box sites for its activity in vivo and is regulated by the MITF-TFE family. J Biol Chem 282:1838-50 (2007). PMID: 17085443.

Huan C et al. Renal Carcinoma-associated Transcription Factors TFE3 and TFEB Are Leukemia Inhibitory Factor-responsive Transcription Activators of E-cadherin. J Biol Chem 280:30225-35 (2005). PubMed: 15994295

We would guarantee this antibody to work in ChIP in accordance with our Abpromise.

I hope this information helps. Please contact us with any other questions.

Read More

Abcam Scientific Support

回复于 May 10 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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