Anti-TFB2M抗体(ab118321)
Key features and details
- Goat polyclonal to TFB2M
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-TFB2M抗体
参阅全部 TFB2M 一抗 -
描述
山羊多克隆抗体to TFB2M -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Mouse TFB2M aa 50-150 (internal sequence) (Cysteine residue).
Database link: NP_032275.2 -
阳性对照
- Mouse and Rat lung lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab118321 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118321于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 289307 Rat
- SwissProt: Q5U2T7 Rat
- Unigene: 96104 Rat
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别名
- N''-adenosyl(rRNA) dimethyltransferase 2 antibody
- S adenosylmethionine 6 N', N' adenosyl(rRNA) dimethyltransferase 2 antibody
- Dimethyladenosine transferase 2 antibody
see all
图片
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Western blot - Anti-TFB2M antibody (ab118321)Anti-TFB2M antibody (ab118321) at 0.5 µg/ml + Rat Lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab118321 尚未被引用在任何文献中。