Anti-Tetranectin抗体[5B7] (ab51883)
![Immunocytochemistry/ Immunofluorescence - Anti-Tetranectin antibody [5B7] (ab51883)](https://www.abcam.cn/ps/products/51/ab51883/Images/ab51883-225498-anti-tetranectin-antibody-5b7-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Tetranectin antibody [5B7] (ab51883)")
Key features and details
- Mouse monoclonal [5B7] to Tetranectin
- Suitable for: ICC/IF, WB
- Reacts with: Cow, Human
- Isotype: IgG2a
概述
-
产品名称
Anti-Tetranectin抗体[5B7]
参阅全部 Tetranectin 一抗 -
描述
小鼠单克隆抗体[5B7] to Tetranectin -
宿主
Mouse -
特异性
ab51883 is specific for amino acids 17-181 of human tetranectin monomer. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Cow, Human -
免疫原
Full length native protein (purified) corresponding to Human Tetranectin. Tetranectin purified from human citrate plasma and coupled to PPD. Boosted before fusion with recombinant tetranectin produced in E. coli.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
5B7 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51883于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
靶标
-
功能
Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis. -
组织特异性
Found in plasma. -
序列相似性
Contains 1 C-type lectin domain. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 515783 Cow
- Entrez Gene: 7123 Human
- Omim: 187520 Human
- SwissProt: Q2KIS7 Cow
- SwissProt: P05452 Human
- Unigene: 476092 Human
-
别名
- C type lectin domain family 3 member B antibody
- C-type lectin domain family 3 member B antibody
- Clec3b antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Tetranectin antibody [5B7] (ab51883)
Immunocytochemistry/Immunofluorescence analysis of human neutrophils labelling Tetranectin (red) with ab51883. Counterstained with DAPI. A - DAPI, B - Tetranectin, C - Overlay.
-
![Western blot - Anti-Tetranectin antibody [5B7] (ab51883)](https://www.abcam.cn/ps/products/51/ab51883/Images/ab51883-63954-anti-tetranectin-antibody-5b7-western-blot.jpg)
Western blot - Anti-Tetranectin antibody [5B7] (ab51883)All lanes : Anti-Tetranectin antibody [5B7] (ab51883) at 5 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Additional bands at: 25 kDa (possible post-translational modification), 55 kDa. We are unsure as to the identity of these extra bands. -
![Western blot - Anti-Tetranectin antibody [5B7] (ab51883)](https://www.abcam.cn/ps/products/51/ab51883/Images/ab51883-225501-anti-tetranectin-antibody-5b7-western-blot.jpg)
Western blot - Anti-Tetranectin antibody [5B7] (ab51883)Lanes 2-3 : Anti-Tetranectin antibody [5B7] (ab51883)
Lane 1 : Marker
Lane 2 : Human serum at 20 µl
Lane 3 : Bovine serum at 20 µl
Predicted band size: 22 kDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab51883 被引用在 5 文献中.
- Wu S et al. Human Papillary and Reticular Fibroblasts Show Distinct Functions on Tumor Behavior in 3D-Organotypic Cultures Mimicking Melanoma and HNSCC. Int J Mol Sci 23:N/A (2022). PubMed: 36232952
- Yan B et al. iTRAQ-based Comparative Serum Proteomic Analysis of Prostate Cancer Patients with or without Bone Metastasis. J Cancer 10:4165-4177 (2019). PubMed: 31413735
- Bæk R et al. Does smoking, age or gender affect the protein phenotype of extracellular vesicles in plasma? Transfus Apher Sci 55:44-52 (2016). PubMed: 27470710
- Richens JL et al. Practical detection of a definitive biomarker panel for Alzheimer's disease; comparisons between matched plasma and cerebrospinal fluid. Int J Mol Epidemiol Genet 5:53-70 (2014). ELISA ; Human . PubMed: 24959311
- Pernemalm M et al. Use of narrow-range peptide IEF to improve detection of lung adenocarcinoma markers in plasma and pleural effusion. Proteomics 9:3414-24 (2009). WB ; Human . PubMed: 19609957