Anti-Tet2抗体(ab124297)
Key features and details
- Rabbit polyclonal to Tet2
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Tet2抗体
参阅全部 Tet2 一抗 -
描述
兔多克隆抗体to Tet2 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse
预测可用于: Human
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免疫原
Synthetic peptide corresponding to Mouse Tet2 aa 1600-1700 conjugated to keyhole limpet haemocyanin.
Database link: Q4JK59 -
阳性对照
- WB: E14Tg2A wild type mouse ES whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124297于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/250. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
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| ICC/IF | (3) |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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WB
1/250. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. -
组织特异性
Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes. -
疾病相关
Note=TET2 is frequently mutated in myeloproliferative disorders (MPD). These constitute a heterogeneous group of disorders, also known as myeloproliferative diseases or myeloproliferative neoplasms (MPN), characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. Included diseases are: essential thrombocythemia, polycythemia vera, primary myelofibrosis (chronic idiopathic myelofibrosis). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
Defects in TET2 are a cause of polycythemia vera (PV) [MIM:263300]. A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly.
Note=TET2 is frequently mutated in systemic mastocytosis; also known as systemic mast cell disease. A condition with features in common with myeloproliferative diseases. It is a clonal disorder of the mast cell and its precursor cells. The clinical symptoms and signs of systemic mastocytosis are due to accumulation of clonally derived mast cells in different tissues, including bone marrow, skin, the gastrointestinal tract, the liver, and the spleen.
Note=TET2 is frequently mutated in myelodysplastic syndromes, a heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites. -
序列相似性
Belongs to the TET family. - Information by UniProt
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数据库链接
- Entrez Gene: 54790 Human
- Entrez Gene: 214133 Mouse
- Omim: 612839 Human
- SwissProt: Q6N021 Human
- SwissProt: Q4JK59 Mouse
- Unigene: 367639 Human
- Unigene: 347816 Mouse
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别名
- FLJ20032 antibody
- KIAA1546 antibody
- MDS antibody
see all
图片
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Western blot - Anti-Tet2 antibody (ab124297)All lanes : Anti-Tet2 antibody (ab124297) at 1/250 dilution
Lane 1 : mES cell lysate
Lane 2 : F9 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 225 kDa why is the actual band size different from the predicted?Western blot: Anti-TET2 antibody (ab124297) staining at 1/250 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab124297 was shown to bind specifically to TET2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Tet2 antibody (ab124297)ab124297 staining Tet2 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab124297 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Western blot - Anti-Tet2 antibody (ab124297)All lanes : Anti-Tet2 antibody (ab124297) at 1/250 dilution (Milk blocking - 1%)
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : WT Mouse ES Cell Lysate (Positive Control for Tet2)
Lane 3 : Tet2 Knockout Mouse ES Cell Lysate (Negative Control)
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Additional bands at: 48 kDa (possible non-specific binding), 65 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab124297 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Secondary antibody - goat anti-rabbit HRP (ab97051)
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Western blot - Anti-Tet2 antibody (ab124297)This image is courtesy of an anonymous abreviewAll lanes : Anti-Tet2 antibody (ab124297) at 1/1000 dilution (Incubated for 12 hours at 4°C)
Lane 1 : Wild type mouse embryonic stem cells with Milk, 1 hour at 21°C
Lane 2 : Tet2 KO clone#1 mouse embryonic stem cells with Milk, 1 hour at 21°C
Lane 3 : Tet2 KO clone#2 mouse embryonic stem cells with Milk, 1 hour at 21°C
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Exposure time: 5 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Tet2 antibody (ab124297)This image is courtesy of an anonymous abreview.ICC/IF image of Anti-Tet2 antibody (ab124297) stained WT and Tet2-/- mouse ES cells. The cells were fixed in PFA, permeabilized using 0.25% Triton X-100, and blocked with 10% BSA for 30 minutes. The cells were then incubated with ab124297 at a 1/400 dilution for 13 hours and 20 minutes at 4oC. The secondary antibody was a Rhodamine Red-X AffiniPure Donkey anti-Rabbit used at a 1/500 dilution.
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Western blot - Anti-Tet2 antibody (ab124297)This image is courtesy of an anonymous abreview.All lanes : Anti-Tet2 antibody (ab124297) at 1/500 dilution
Lane 1 : Wild-type mouse embryonic stem cells
Lane 2 : Tet2-/- mouse embryonic stem cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit (whole molecules) IgG HRP at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Tet2 antibody (ab124297)This image is courtesy of an abreview from Joe Segal.ICC/IF image of Anti-Tet2 antibody (ab124297) stained D3 mouse ES cells. The cells were fixed in 4% PFA, permeabilized using 0.1% Triton X-100, and blocked with 1% Goat serum, 0.1% BSA in PBS for 30 minutes. The cells were then incubated with ab124297 at a 1/100 dilution for 2 hours at RT. The secondary antibody was a Goat polyclonal Secondary Antibody to Rabbit IgG – H&L Alexa Fluor 488 (ab150077) used at a 1/200 dilution.
Secondary antibody - goat anti-rabbit Alexa 488 (ab150077)
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab124297 被引用在 36 文献中.
- Liu Y et al. TET2 is recruited by CREB to promote Cebpb, Cebpa, and Pparg transcription by facilitating hydroxymethylation during adipocyte differentiation. iScience 26:108312 (2023). PubMed: 38026190
- Morante-Palacios O et al. Coordinated glucocorticoid receptor and MAFB action induces tolerogenesis and epigenome remodeling in dendritic cells. Nucleic Acids Res 50:108-126 (2022). PubMed: 34893889
- Chrysanthou S et al. The DNA dioxygenase Tet1 regulates H3K27 modification and embryonic stem cell biology independent of its catalytic activity. Nucleic Acids Res 50:3169-3189 (2022). PubMed: 35150568
- Ma L et al. Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis. Sci Adv 8:eabm3470 (2022). PubMed: 35235365
- Huang X et al. A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell Rep 39:110928 (2022). PubMed: 35675764