Anti-TET1抗体- C-terminal (ab191698)
Key features and details
- Rabbit polyclonal to TET1 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TET1抗体- C-terminal
参阅全部 TET1 一抗 -
描述
兔多克隆抗体to TET1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human TET1 (C terminal). The exact sequence is proprietary. 18 amino acid peptide. The immunogen is located with amino acids 2030 - 2080.
Database link: Q8NFU7 -
阳性对照
- Western blot: Human, mouse and rat testis; Human bladder; Mouse and rat kidney; Mouse and rat thymus; Mouse stomach; K562, A431 whole cell lysates. IHC-P: Human and mouse testis, mouse kidney and rat liver tissues. ICC/IF: HeLa and EL4 cell lines.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab191698于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 235 kDa.
Recommend for human and mouse and rat. Please see image legend for details of the Western blot protocol. Blocking in milk and incubation of ab191698 for 1 hour at room temperature. |
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Recommend for human and mouse. Please see image legends for more protocol details. |
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| ICC/IF |
Use a concentration of 20 µg/ml.
Recommend for human and mouse. Please see image legends for more protocol details. |
| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 235 kDa. Recommend for human and mouse and rat. Please see image legend for details of the Western blot protocol. Blocking in milk and incubation of ab191698 for 1 hour at room temperature. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommend for human and mouse. Please see image legends for more protocol details. |
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ICC/IF
Use a concentration of 20 µg/ml. Recommend for human and mouse. Please see image legends for more protocol details. |
靶标
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功能
Dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays a role in embryonic stem (ES) cell maintenance and inner cell mass (ICM) cell specification, possibly by participating in DNA demethylation. Specifically binds 5mC, a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. 5mC is present in ES cells and is enriched in the brain, especially in Purkinje neurons. The clear function of hmC is still unclear but it could constitute an intermediate component in cytosine demethylation. A role of hmC in DNA demethylation is supported by TET1 function in ES cell maintenance, which is required to prevent NANOG hypermethylation and maintain NANOG expression in ES cells. -
组织特异性
Expressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain. -
疾病相关
Note=A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'. -
序列相似性
Belongs to the TET family.
Contains 1 CXXC-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 80312 Human
- Entrez Gene: 52463 Mouse
- Entrez Gene: 309902 Rat
- Omim: 607790 Human
- SwissProt: Q8NFU7 Human
- SwissProt: Q3URK3 Mouse
- Unigene: 567594 Human
- Unigene: 708977 Human
see all -
别名
- bA119F7.1 antibody
- CXXC 6 antibody
- CXXC finger 6 antibody
see all
图片
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Western blot - Anti-TET1 antibody - C-terminal (ab191698)All lanes : Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/ml
Lane 1 : Mouse testis
Lane 2 : Rat testis
Lane 3 : Human testis
Lane 4 : Human bladder
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 235 kDa
Additional bands at: 55-72 kDa (possible non-specific binding)
Exposure time: 1 minute8% SDS gel
Running condition: 130V, 1.5 hours.
Transfer condition: Wet, 250mA, 2 hrs (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight.
Primary antibody incubation: room temperature for 1 hour in 5% NFDM/TBST.
Secondary antibody incubation: room temperature for 1 hour.
Washing condition: 5 mL TBST, 4 x 5 minutes.
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Western blot - Anti-TET1 antibody - C-terminal (ab191698)All lanes : Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/ml
Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : abbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 235 kDa
Observed band size: 235 kDaWestern blot analysis labeling TET1 with ab191698 at 1 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.
Running condition: 8% gel, 130V for 1.5 hrs.
Transfer condition: Wet, 250mA, 2 hrs (NC membrane).
Blocking condition: 5% non-fat milk in TBS at 4°C overnight.
Washing condition: 5ml TBST, 4 x 5 min.
Exposure system: GE ECL 1 min.
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Western blot - Anti-TET1 antibody - C-terminal (ab191698)All lanes : Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/ml
Lane 1 : Mouse kidney
Lane 2 : Mouse thymus
Lane 3 : Mouse stomach
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 235 kDa
Observed band size: 235 kDaWestern blot analysis labeling TET1 with ab191698 at 1 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.
Running condition: 8% gel, 130V for 1.5 hrs.
Transfer condition: Wet, 250mA, 2 hrs (NC membrane).
Blocking condition: 5% non-fat milk in TBS at 4°C overnight.
Washing condition: 5ml TBST, 4 x 5 min.
Exposure system: GE ECL 1 min.
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Western blot - Anti-TET1 antibody - C-terminal (ab191698)All lanes : Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/ml
Lane 1 : Rat kidney
Lane 2 : Rat thymus
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 235 kDa
Observed band size: 235 kDaWestern blot analysis labeling TET1 with ab191698 at 1 μg/mL in 5% NFDM/TBST, followed by secondary antibody, incubated at room temperature for 1hr.
Running condition: 8% gel, 130V for 1.5 hrs.
Transfer condition: Wet, 250mA, 2 hrs (NC membrane).
Blocking condition: 5% non-fat milk in TBS at 4°C overnight.
Washing condition: 5ml TBST, 4 x 5 min.
Exposure system: GE ECL 10 sec.
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Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody - C-terminal (ab191698)Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) HeLa cells labeling TET1 with ab191698 at 20 µg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 3 x 3 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody - C-terminal (ab191698)Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) EL4 cells (mouse T-lymphocyte cell line) labeling TET1 with ab191698 at 20 µg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on EL4 cell line. The nuclear counter stain is DAPI (blue).
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 3 x 3 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TET1 with ab191698 at 1 µg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on human testis is observed. DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TET1 with ab191698 at 1 µg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, Room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TET1 with ab191698 at 1 µg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, Room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling TET1 with ab191698 at 1 µg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, Room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling TET1 with ab191698 at 1 µg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on mouse testis is observed. DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (43)
ab191698 被引用在 43 文献中.
- Shi G et al. DNA hypomethylation promotes learning and memory recovery in a rat model of cerebral ischemia/reperfusion injury. Neural Regen Res 18:863-868 (2023). PubMed: 36204855
- Toniyan KA et al. Endometriosis of the Cervix: A Rare Clinical Case with the Possibility of Comparing the Eutopic and Ectopic Endometrium at the Cellular Level. Int J Mol Sci 24:N/A (2023). PubMed: 36768508
- Zeng Z et al. Overexpression of OASL upregulates TET1 to induce aberrant activation of CD4+ T cells in systemic sclerosis via IRF1 signaling. Arthritis Res Ther 24:50 (2022). PubMed: 35183246
- Kong FC et al. A novel epigenetic marker, Ten-eleven translocation family member 2 (TET2), is identified in the intractable epileptic brain and regulates ATP binding cassette subfamily B member 1 (ABCB1) in the blood-brain barrier. Bioengineered 13:6638-6649 (2022). PubMed: 35235761
- Wang L et al. Dexmedetomidine prevents cardiomyocytes from hypoxia/reoxygenation injury via modulating tetmethylcytosine dioxygenase 1-mediated DNA demethylation of Sirtuin1. Bioengineered 13:9369-9386 (2022). PubMed: 35387565