Anti-TET1抗体(ab272900)
Key features and details
- Rabbit polyclonal to TET1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-TET1抗体
参阅全部 TET1 一抗 -
描述
兔多克隆抗体to TET1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human TET1 (internal sequence). The exact sequence is proprietary.
Database link: Q8NFU7 -
阳性对照
- WB: HEK-293T, A431, HeLa, HepG2 cells. IHC-P: A549 xenograft, HeLa cells, Human normal prostate tissue. ICC/IF: HEK-293T cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272900于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| IHC-P |
1/100 - 1/1000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. |
|
ICC/IF
1/100 - 1/1000. |
靶标
-
功能
Dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays a role in embryonic stem (ES) cell maintenance and inner cell mass (ICM) cell specification, possibly by participating in DNA demethylation. Specifically binds 5mC, a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. 5mC is present in ES cells and is enriched in the brain, especially in Purkinje neurons. The clear function of hmC is still unclear but it could constitute an intermediate component in cytosine demethylation. A role of hmC in DNA demethylation is supported by TET1 function in ES cell maintenance, which is required to prevent NANOG hypermethylation and maintain NANOG expression in ES cells. -
组织特异性
Expressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain. -
疾病相关
Note=A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'. -
序列相似性
Belongs to the TET family.
Contains 1 CXXC-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 80312 Human
- Entrez Gene: 52463 Mouse
- Entrez Gene: 309902 Rat
- Omim: 607790 Human
- SwissProt: Q8NFU7 Human
- SwissProt: Q3URK3 Mouse
- Unigene: 567594 Human
- Unigene: 708977 Human
see all -
别名
- bA119F7.1 antibody
- CXXC 6 antibody
- CXXC finger 6 antibody
see all
图片
-
Western blot - Anti-TET1 antibody (ab272900)All lanes : Anti-TET1 antibody (ab272900) at 1/5000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : DDDDK-human TET1 transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody (ab272900)Paraffin embedded A549 xenograft tissue stained for TET1 using ab272900 at 1/250 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
-
Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody (ab272900)4% paraformaldehyde-fixed Mock and transfected HEK-293T cells stained for TET1 (green) using ab272900 at 1/1000 dilution in ICC/IF analysis. Co-stained with Hoechest33342 (Blue).
-
Western blot - Anti-TET1 antibody (ab272900)All lanes : Anti-TET1 antibody (ab272900) at 1/2000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody (ab272900)Paraffin embedded HeLa xenograft tissue stained for TET1 using ab272900 at 1/1000 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
-
Western blot - Anti-TET1 antibody (ab272900)All lanes : Anti-TET1 antibody (ab272900) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa nuclear extract lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody (ab272900)Paraffin embedded Human normal prostate tissue stained for TET1 using ab272900 at 1/1000 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab272900 被引用在 1 文献中.
- Tang X et al. Upregulation of CRABP2 by TET1-mediated DNA hydroxymethylation attenuates mitochondrial apoptosis and promotes oxaliplatin resistance in gastric cancer. Cell Death Dis 13:848 (2022). PubMed: 36195596