重组Anti-Tenascin C抗体[EPR4219] - Low endotoxin,Azide free
Anti-Tenascin C antibody [EPR4219] - Low endotoxin, Azide free
- RabMAb
- Recombinant
- 了解详情
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(5 Publications)
Rabbit Recombinant Monoclonal Tenascin C antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
查看别名
HXB, TNC, Tenascin, TN, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, TN-C
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] - Low endotoxin, Azide free (AB215369)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (ab93684). Hematoxylin was used to counterstain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID : 1372043) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab108930).
- IHC-Fr
Lab
Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] - Low endotoxin, Azide free (AB215369)
Immunohistochemistry (Frozen sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077 an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on the molecular layer of rat cerebellar cortex (PMID : 1372043) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab108930).
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] - Low endotoxin, Azide free (AB215369)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain. Staining on the molecular layer of rat cerebellar cortex (PMID : 1372043) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab108930).
- IHC-Fr
Lab
Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] - Low endotoxin, Azide free (AB215369)
Immunohistochemistry (Frozen sections) analysis of mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077 an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID : 1372043) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab108930).
- IHC-Fr
Lab
Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] - Low endotoxin, Azide free (AB215369)
Immunohistochemistry (Frozen sections) analysis of mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077 an AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID : 9822997; PMID : 19586317; PMID : 24778247) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab108930).
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] - Low endotoxin, Azide free (AB215369)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain.
Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID : 9822997; PMID : 19586317; PMID : 24778247) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab108930).
不同偶联物与剂型 (6)
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578 PE
PE Anti-Tenascin C antibody [EPR4219]
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Tenascin C antibody [EPR4219]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Tenascin C antibody [EPR4219]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Tenascin C antibody [EPR4219]
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660 APC
APC Anti-Tenascin C antibody [EPR4219]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Tenascin C antibody [EPR4219]
反应性数据
产品详情
ab215369 is the carrier-free version of ab108930.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The large hexamer known as Tenascin C plays a significant role in modulating cell adhesion migration and proliferation especially during developmental processes and injury responses. It incorporates into the existing extracellular matrix as a loose network which provides elasticity and supports cellular activities. This feature allows Tenascin C to interact with other matrix molecules and cell surface receptors to regulate tissue morphogenesis.
Pathways
Tenascin C actively participates in the Wnt and TGF-beta signaling pathways important for controlling cellular behavior in development and regeneration. It associates with integrins and glycoproteins to influence cell responses to signaling events in these pathways. By modulating these interactions Tenascin C can impact processes such as epithelial-to-mesenchymal transition and angiogenesis affecting tissue architecture and function.
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (5)
Recent publications for all applications. Explore the full list and refine your search
Materials today. Bio 31:101500 PubMed39944530
2025
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in immunology 12:791907 PubMed34956224
2021
Applications
Unspecified application
Species
Unspecified reactive species
Journal of the American Society of Nephrology : JA 25:953-66 PubMed24436469
2014
Applications
Unspecified application
Species
Unspecified reactive species
The American journal of pathology 184:765-77 PubMed24384131
2014
Applications
IHC
Species
Rat
The Journal of biological chemistry 288:29760-70 PubMed23950180
2013
Applications
IHC, WB
Species
Mouse, Mouse
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