Anti-Tenascin C 抗体 [EPR4219]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (AB108930)

Immunohistochemistry (Frozen sections) analysis of mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077 an AlexaFluor^® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.

Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID : 9822997; PMID : 19586317; PMID : 24778247) is observed.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (AB108930)

Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labelling tenascin C with ab108930 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a ChromoMap DAB kit and anti-rabbit HQ and anti-HQ HRP detection. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab108930 anti-Tenascin C antibody [EPR4219]was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (AB108930)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (ab93684). Hematoxylin was used to counterstain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID : 1372043) is observed.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (AB108930)

Immunohistochemistry (Frozen sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077 an AlexaFluor^® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.

Positive staining on the molecular layer of rat cerebellar cortex (PMID : 1372043) is observed.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (AB108930)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain. Staining on the molecular layer of rat cerebellar cortex (PMID : 1372043) is observed.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (AB108930)

Immunohistochemistry (Frozen sections) analysis of mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077 an AlexaFluor^® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.

Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID : 1372043) is observed.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (AB108930)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain.

Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID : 9822997; PMID : 19586317; PMID : 24778247) is observed.

• WB

Lab

Western blot - Anti-Tenascin C antibody [EPR4219] (AB108930)

Western blot : Anti-TNC antibody [EPR4219] (ab108930) staining at 1/500 dilution, shown in black; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab108930 was shown to bind specifically to TNC. A band was observed at 122 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in TNC knockout cell line. To generate this image, wild-type and TNC knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 2 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 5 seconds exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/500 dilution

Lane 1:

Wild-type U-2 OS BFA (5 ug/mL, 6 h) cell lysate at 40 µg

Lane 2:

Wild-type U-2 OS BFA (0 ug/mL, 6 h) cell lysate at 40 µg

Lane 3:

TNC knockout U-2 OS BFA (5 ug/mL, 6 h) cell lysate at 40 µg

Lane 4:

TNC knockout U-2 OS BFA (0 ug/mL, 6 h) cell lysate at 40 µg

Lane 5:

U-87 MG Treated BFA (5 ug/mL, 6 h) cell lysate at 10 µg

Lane 6:

U-87 MG Control BFA (0 ug/mL, 6 h) cell lysate at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

false

• WB

Lab

Western blot - Anti-Tenascin C antibody [EPR4219] (AB108930)

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

Liver is negative control (PMID : 1717349). The molecular weight observed is consistent with what has been described in the literature (PMID : 10462531).

All lanes:

Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

Lane 1:

Human fetal brain at 20 µg

Lane 2:

Human liver at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 241 kDa,33 kDa,45 kDa

Observed band size: 250 kDa,42 kDa,45 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-Tenascin C antibody [EPR4219] (AB108930)

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 10462531).

All lanes:

Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

All lanes:

U87-MG (human glioblastoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 241 kDa

Observed band size: 250 kDa

false

Exposure time: 3min

• WB

Unknown

Western blot - Anti-Tenascin C antibody [EPR4219] (AB108930)

All lanes:

Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

All lanes:

Human fetal brain lysate at 10 µg

Predicted band size: 241 kDa

false

• WB

Lab

Western blot - Anti-Tenascin C antibody [EPR4219] (AB108930)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure Time :
Lane 1 : 15 seconds
Lane 2 : 30 seconds

The molecular weight observed is consistent with what has been described in the literature (PMID : 10462531).

All lanes:

Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

Lane 1:

Postnatal (P0) mouse cerebellum at 20 µg

Lane 2:

Postnatal (P0) rat brain at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 241 kDa

Observed band size: 250 kDa

false