Anti-TEM8/ATR抗体(ab21269)
Key features and details
- Rabbit polyclonal to TEM8/ATR
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TEM8/ATR抗体
参阅全部 TEM8/ATR 一抗 -
描述
兔多克隆抗体to TEM8/ATR -
宿主
Rabbit -
特异性
This antibody will recognize only the largest isoform of TEM8/ATR.
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经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human TEM8/ATR (C terminal).
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阳性对照
- WB: K562 cell lysates, Wild-type HeLa cell lysate, U-2 OS cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
This antibody is ion exchange chromatography purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 63 kDa.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 63 kDa. |
靶标
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功能
Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. -
组织特异性
Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells. -
疾病相关
Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
序列相似性
Belongs to the ATR family.
Contains 1 VWFA domain. -
结构域
Binding to PA occurs through the VWA domain. -
细胞定位
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia. - Information by UniProt
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数据库链接
- Entrez Gene: 84168 Human
- Omim: 606410 Human
- SwissProt: Q9H6X2 Human
- Unigene: 165859 Human
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别名
- Anthrax toxin receptor 1 antibody
- ANTR1_HUMAN antibody
- Antxr1 antibody
see all
图片
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Western blot - Anti-TEM8/ATR antibody (ab21269)All lanes : Anti-TEM8/ATR antibody (ab21269) at 1 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ANTXR1 knockout HeLa cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-TEM8/ATR antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab21269 was shown to bind specifically to TEM8/ATR. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in ANTXR1 knockout cell line ab265077 (knockout cell lysate ab257350). To generate this image, wild-type and ANTXR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Western blot - Anti-TEM8/ATR antibody (ab21269)Lane 1 : Anti-TEM8/ATR antibody (ab21269) at 0.5 µg/ml
Lane 2 : Anti-TEM8/ATR antibody (ab21269) at 1 µg/ml
Lane 3 : Anti-TEM8/ATR antibody (ab21269) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 63 kDaWestern blot analysis of TEM8/ATR in K562 cell lysates with ab21269 at (A) 0.5, (B) 1 and (C) 2µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody (ab21269)ab21269 at 2µg/ml staining TEM8/ATR in K562 cells by ICC/IF
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab21269 被引用在 3 文献中.
- Cryan LM et al. Capillary morphogenesis gene 2 (CMG2) mediates growth factor-induced angiogenesis by regulating endothelial cell chemotaxis. Angiogenesis 25:397-410 (2022). PubMed: 35212873
- Zhang Q et al. Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma. Cells 8:N/A (2019). PubMed: 31121964
- Hu K et al. Cell autonomous ANTXR1-mediated regulation of extracellular matrix components in primary fibroblasts. Matrix Biol 62:105-114 (2017). PubMed: 28011198