Anti-TEM1抗体(ab67273)
Key features and details
- Rabbit polyclonal to TEM1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TEM1抗体
参阅全部 TEM1 一抗 -
描述
兔多克隆抗体to TEM1 -
宿主
Rabbit -
特异性
ab67273 recognizes both isoforms of TEM1. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
A 14 amino acid synthetic peptide derived from near the carboxy terminus of the human TEM1.
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阳性对照
- WB: Human Colon tissue lysate. HEK-293, A431, A549, and CaCo-2 cell lysates; IHC-P: Human Colon tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67273于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46, 90 kDa (predicted molecular weight: 46, 81 kDa).
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46, 90 kDa (predicted molecular weight: 46, 81 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
May play a role in tumor angiogenesis. -
组织特异性
Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts. -
序列相似性
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain. -
翻译后修饰
O-glycosylated with sialylated oligosaccharides.
May be N-glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 57124 Human
- Omim: 606064 Human
- SwissProt: Q9HCU0 Human
- Unigene: 195727 Human
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别名
- CD164 sialomucin like 1 antibody
- CD164L1 antibody
- CD248 antibody
see all
图片
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Western blot - Anti-TEM1 antibody (ab67273)All lanes : Anti-TEM1 antibody (ab67273) at 2 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 46, 81 kDa
Observed band size: 46, 90 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-TEM1 antibody (ab67273)Immunofluorescent analysis of 4% paraformaldehydefixed Human Colon tissue labeling TEM-1 with ab67273 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Western blot - Anti-TEM1 antibody (ab67273)Lane 1 : Anti-TEM1 antibody (ab67273) at 0.5 µg/ml
Lane 2 : Anti-TEM1 antibody (ab67273) at 1 µg/ml
All lanes : Human colon tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti rabbit secondary antibody
Predicted band size: 46, 81 kDa
Observed band size: 50,80 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM1 antibody (ab67273)ab67273 at 2.5 μg/ml.in staining of TEM1 in human colon tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab67273 被引用在 4 文献中.
- Wu C et al. TEM1 up-regulates MMP-2 and promotes ECM remodeling for facilitating invasion and migration of uterine sarcoma. Discov Oncol 14:5 (2023). PubMed: 36639546
- Liu S et al. Antibody-drug conjugates targeting CD248 inhibits liver fibrosis through specific killing on myofibroblasts. Mol Med 28:37 (2022). PubMed: 35317721
- de Oliveira TD et al. Stromal cells support the survival of human primary chronic lymphocytic leukemia (CLL) cells through Lyn-driven extracellular vesicles. Front Med (Lausanne) 9:1059028 (2022). PubMed: 36714146
- Kendal AR et al. Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon. Sci Rep 10:13939 (2020). PubMed: 32883960