Anti-TEAD4抗体(ab97460)

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab97460 and ab48921 by reducing the background and enhancing the specific binding : 1) I would suggest to load less protein sample on the gel, 10 or 20µg. 2) I would recommend to use the same blocking agent all along the protocol. 3) When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend switching to this instead of milk. Some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands. An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : Click here for the western blot image using ab9385 (or use the following: www.abcam.com/ab9385). 4) I would suggest to block the membrane for 1 hour at room temperature. 5) An overnight incubation at 4°C of the primary diluted in the blocking solution is commonly recommended. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.