Anti-TEA domain family member 2/ETF抗体[404C5a] (ab54374)
![Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374)](https://www.abcam.cn/ps/products/54/ab54374/Images/ab54374-67217-anti-tea-domain-family-member-2-etf-antibody-404c5a-western-blot.gif "Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374)")
Key features and details
- Mouse monoclonal [404C5a] to TEA domain family member 2/ETF
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
-
产品名称
Anti-TEA domain family member 2/ETF抗体[404C5a]
参阅全部 TEA domain family member 2/ETF 一抗 -
描述
小鼠单克隆抗体[404C5a] to TEA domain family member 2/ETF -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human TEA domain family member 2/ETF.
Database link: Q15562 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab54374 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
404C5a -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54374于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. |
靶标
-
功能
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif. -
序列相似性
Contains 1 TEA DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
-
别名
- Embryonic TEA domain containing factor antibody
- Etdf antibody
- ETEF 1 antibody
see all
图片
-
Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374)Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374) + immunizing recombinant protein
Predicted band size: 49 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen.
数据表及文件
-
Datasheet download
文献 (0)
ab54374 尚未被引用在任何文献中。
