Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-TdT抗体[EPR2976Y] (ab76544)

Submit a review Submit a question References (2)
Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
  • Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (ab76544)
  • Flow Cytometry (Intracellular) - Anti-TdT antibody [EPR2976Y] (ab76544)
  • Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] (ab76544)
  • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
  • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
  • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
  • Anti-TdT antibody [EPR2976Y] (ab76544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2976Y] to TdT
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TdT抗体[EPR2976Y]
    参阅全部 TdT 一抗

  • 描述

    兔单克隆抗体[EPR2976Y] to TdT

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IP, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human TdT (C terminal).

  • 阳性对照

    • Human thymus and Jurkat lysates.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR2976Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76544于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/20.
WB
1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP
1/20.
ICC/IF
1/100 - 1/250.
说明
Flow Cyt (Intra)
1/20.
WB
1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP
1/20.
ICC/IF
1/100 - 1/250.

靶标

  • 功能

    Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

  • 序列相似性

    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Deoxynucleotidyltransferase terminal antibody
      • DNA nucleotidylexotransferase antibody
      • DNTT antibody
      • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
      • TDT antibody
      • TDT_HUMAN antibody
      • Terminal addition enzyme antibody
      • Terminal deoxynucleotidyltransferase antibody
      • Terminal deoxyribonucleotidyltransferase antibody
      • Terminal transferase antibody
      see all

    图片

    • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
      Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
      Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution (purified) + Human fetal thymus lysates at 15 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 58 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (ab76544)
      Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (ab76544)

      ab76544 (purified) at 1:20 dilution (2μg) immunoprecipitating TdT in Jurkat (human acute T cell leukemia) whole cell lysate.

      Lane 1 (input): Jurkat (human acute T cell leukemia) whole cell lysate 10μg
      Lane 2 (+): ab76544 & Jurkat (human acute T cell leukemia) whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76544 in Jurkat (human acute T cell leukemia) whole cell lysate

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-TdT antibody [EPR2976Y] (ab76544)
      Flow Cytometry (Intracellular) - Anti-TdT antibody [EPR2976Y] (ab76544)

      Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TdT with purified ab76544 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] (ab76544)
      Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] (ab76544)

      Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling TdT with Purified ab76544 at 1:100 dilution (1.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
      Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
      All lanes : Anti-TdT antibody [EPR2976Y] (ab76544) at 1/2000 dilution (purified)

      Lane 1 : Jurkat (human acute T cell leukemia) whole cell lysates
      Lane 2 : Mouse thymus lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 58 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
      Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
      Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution (purified) + Rat thymus lysates at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 58 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
      Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)
      All lanes : Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution (unpurified)

      Lane 1 : Human thymus lysate
      Lane 2 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa
      Additional bands at: 24 kDa, 35 kDa. We are unsure as to the identity of these extra bands.

    • Anti-TdT antibody [EPR2976Y] (ab76544)
      Anti-TdT antibody [EPR2976Y] (ab76544)

    文献 (2)

    发表研究结果有使用 ab76544？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab76544 被引用在 2 文献中.

    • Laporte E  et al. Decoding the activated stem cell phenotype of the neonatally maturing pituitary. Elife 11:N/A (2022). PubMed: 35699412
    • Aït Ghezali L  et al. Induction of endoplasmic reticulum calcium pump expression during early leukemic B cell differentiation. J Exp Clin Cancer Res 36:87 (2017). PubMed: 28651627

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab76544.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com