重组Anti-TDRD3抗体[RAB-C419] (ab317727)
![Immunocytochemistry/ Immunofluorescence - Anti-TDRD3 antibody [RAB-C419] (ab317727)](https://www.abcam.cn/ps/products/317/ab317727/Images/ab317727-3-anti-tdrd3-antibody-rab-c419-immunocytochemistry-hela-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TDRD3 antibody [RAB-C419] (ab317727)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [RAB-C419] to TDRD3
- Suitable for: WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
概述
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产品名称
Anti-TDRD3抗体[RAB-C419]
参阅全部 TDRD3 一抗 -
描述
人单克隆抗体[RAB-C419] to TDRD3 -
宿主
Human -
特异性
This antibody recognizes TDRD3 (Tudor domain-containing protein 3). It binds to a folded domain, amino acids 1-455.
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经测试应用
适用于: WB, Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human TDRD3 aa 550-650. N-terminal tag
Database link: Q9H7E2 -
阳性对照
- Flow Cyt (Intra): HeLa cells. ICC/IF: HeLa cells. WB: Human cerebellum, skeletal muscle, heart, placenta, testes and kidney tissue lysate.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified by Immobilized Metal Affinity Chromatography. -
克隆
单克隆 -
克隆编号
RAB-C419 -
同种型
unknown -
轻链类型
kappa -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317727于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins. -
组织特异性
Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
序列相似性
Contains 1 Tudor domain.
Contains 1 UBA domain. -
结构域
The Tudor domain specifically recognizes and binds asymmetric dimethylation of histone H3 'Arg-17' (H3R17me2a) and histones H4 'Arg-3', 2 tags for epigenetic transcriptional activation. -
细胞定位
Cytoplasm. Nucleus. Predominantly cytoplasmic. Associated with actively translating polyribosomes and with mRNA stress granules. - Information by UniProt
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数据库链接
- Entrez Gene: 81550 Human
- Omim: 614392 Human
- SwissProt: Q9H7E2 Human
- Unigene: 525061 Human
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别名
- 4732418C03Rik antibody
- 6720468N18 antibody
- FLJ21007 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-TDRD3 antibody [RAB-C419] (ab317727)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of ab317727 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nucleoli, nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom primary antibody/secondary, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
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![Western blot - Anti-TDRD3 antibody [RAB-C419] (ab317727)](https://www.abcam.cn/ps/products/317/ab317727/Images/ab317727-2-anti-tdrd3-antibody-rab-c419-western-blot-cerebellum-skeletal-muscle-heart-placenta-testis-kidney-human.jpg)
Western blot - Anti-TDRD3 antibody [RAB-C419] (ab317727)Lane 1 : Chimeric rabbit version of ab317727 at 1 µg/ml
Lanes 2-3 & 5 : Chimeric rabbit version of ab317727 at 0.3 µg/ml
Lanes 4 & 6 : Chimeric rabbit version of ab317727 at 0.1 µg/ml
Lane 1 : Human cerebellum tissue lysate (35ug protein in RIPA buffer)
Lane 2 : Human skeletal muscle tissue lysate (35ug protein in RIPA buffer)
Lane 3 : Human heart tissue lysate (35ug protein in RIPA buffer)
Lane 4 : Human placenta tissue lysate (35ug protein in RIPA buffer)
Lane 5 : Human testes tissue lysate (35ug protein in RIPA buffer)
Lane 6 : Human kidney tissue lysate (35ug protein in RIPA buffer)
Secondary
All lanes : Anti-rabbit secondary antibodyA primary incubation of 1h was used and protein was detected by chemiluminescence.
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![Flow Cytometry (Intracellular) - Anti-TDRD3 antibody [RAB-C419] (ab317727)](https://www.abcam.cn/ps/products/317/ab317727/Images/ab317727-1-anti-tdrd3-antibody-rab-c419-flow-cytometry-intracellular-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-TDRD3 antibody [RAB-C419] (ab317727)Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of ab317727 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317727 尚未被引用在任何文献中。