Anti-TDP1抗体(ab4166)
Key features and details
- Rabbit polyclonal to TDP1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-TDP1抗体
参阅全部 TDP1 一抗 -
描述
兔多克隆抗体to TDP1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Recombinant fragment
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
Abcam recommends blocking with 1-3% Milk. |
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa). Abcam recommends blocking with 1-3% Milk. |
靶标
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功能
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. -
组织特异性
Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. -
疾病相关
Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy -
序列相似性
Belongs to the tyrosyl-DNA phosphodiesterase family. -
翻译后修饰
Phosphorylated on serine and/or threonine residues, but not on tyrosine residues. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 55775 Human
- Entrez Gene: 104884 Mouse
- Entrez Gene: 314380 Rat
- Omim: 607198 Human
- SwissProt: Q9NUW8 Human
- SwissProt: Q8BJ37 Mouse
- SwissProt: Q4G056 Rat
- Unigene: 209945 Human
see all -
别名
- AI838772 antibody
- AW493413 antibody
- FLJ11090 antibody
see all
图片
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Western blot - Anti-TDP1 antibody (ab4166)Anti-TDP1 antibody (ab4166) at 1 µg/ml (blocked with 3% milk) + Jurkat nuclear extract lysate (ab14844) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes -
Western blot - Anti-TDP1 antibody (ab4166)This image is courtesy of Keith Caldecott, University of SussexLane 1 : Molecular weight markers
Lanes 2-5 : Anti-TDP1 antibody (ab4166) at 1/1000 dilution
Lane 2 : 1 ng purified recombinant human TDP1
Lane 3 : 3 ng purified recombinant human TDP1
Lane 4 : 6 ng purified recombinant human TDP1
Lane 5 : 6 micrograms total protein from MCF7 human cell extract
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDaab4166 was incubated for 1 hr at room temp.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab4166 被引用在 24 文献中.
- Álvarez-Quilón A et al. Endogenous DNA 3' Blocks Are Vulnerabilities for BRCA1 and BRCA2 Deficiency and Are Reversed by the APE2 Nuclease. Mol Cell 78:1152-1165.e8 (2020). PubMed: 32516598
- Saha S et al. DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein Crosslinks. Cell Rep 33:108569 (2020). PubMed: 33378676
- Ghosh A et al. SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy. Sci Adv 5:eaax9778 (2019). PubMed: 31723605
- Rehman I et al. PRMT5-mediated arginine methylation of TDP1 for the repair of topoisomerase I covalent complexes. Nucleic Acids Res 46:5601-5617 (2018). PubMed: 29718323
- Meisenberg C et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res 45:1159-1176 (2017). WB ; Human . PubMed: 28180300