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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Topoisomerases

Anti-TDP1抗体(ab4166)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (24)

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Western blot - Anti-TDP1 antibody (ab4166)
  • Western blot - Anti-TDP1 antibody (ab4166)

Key features and details

  • Rabbit polyclonal to TDP1
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

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概述

  • 产品名称

    Anti-TDP1抗体
    参阅全部 TDP1 一抗
  • 描述

    兔多克隆抗体to TDP1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human, Recombinant fragment
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Topoisomerases
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TDP1 protein (ab131921)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4166于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (3)
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).

Abcam recommends blocking with 1-3% Milk.

说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).

Abcam recommends blocking with 1-3% Milk.

靶标

  • 功能

    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
  • 组织特异性

    Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
  • 疾病相关

    Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy
  • 序列相似性

    Belongs to the tyrosyl-DNA phosphodiesterase family.
  • 翻译后修饰

    Phosphorylated on serine and/or threonine residues, but not on tyrosine residues.
  • 细胞定位

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession Q9NUW8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 55775 Human
    • Entrez Gene: 104884 Mouse
    • Entrez Gene: 314380 Rat
    • Omim: 607198 Human
    • SwissProt: Q9NUW8 Human
    • SwissProt: Q8BJ37 Mouse
    • SwissProt: Q4G056 Rat
    • Unigene: 209945 Human
    • Unigene: 312323 Mouse
    • Unigene: 487464 Mouse
    • Unigene: 488658 Mouse
    • Unigene: 12661 Rat
    see all
  • 别名

    • AI838772 antibody
    • AW493413 antibody
    • FLJ11090 antibody
    • MGC104252 antibody
    • MGC112732 antibody
    • RP24-311F12.2 antibody
    • SCAN1 antibody
    • TDP1 antibody
    • TYDP antibody
    • TYDP1_HUMAN antibody
    • Tyr-DNA phosphodiesterase 1 antibody
    • Tyrosyl-DNA phosphodiesterase 1 antibody
    see all

图片

  • Western blot - Anti-TDP1 antibody (ab4166)
    Western blot - Anti-TDP1 antibody (ab4166)
    Anti-TDP1 antibody (ab4166) at 1 µg/ml (blocked with 3% milk) + Jurkat nuclear extract lysate (ab14844) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes
  • Western blot - Anti-TDP1 antibody (ab4166)
    Western blot - Anti-TDP1 antibody (ab4166)This image is courtesy of Keith Caldecott, University of Sussex
    Lane 1 : Molecular weight markers
    Lanes 2-5 : Anti-TDP1 antibody (ab4166) at 1/1000 dilution

    Lane 2 : 1 ng purified recombinant human TDP1
    Lane 3 : 3 ng purified recombinant human TDP1
    Lane 4 : 6 ng purified recombinant human TDP1
    Lane 5 : 6 micrograms total protein from MCF7 human cell extract


    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    ab4166 was incubated for 1 hr at room temp.

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (24)

发表研究结果有使用 ab4166?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab4166 被引用在 24 文献中.

  • Álvarez-Quilón A  et al. Endogenous DNA 3' Blocks Are Vulnerabilities for BRCA1 and BRCA2 Deficiency and Are Reversed by the APE2 Nuclease. Mol Cell 78:1152-1165.e8 (2020). PubMed: 32516598
  • Saha S  et al. DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein Crosslinks. Cell Rep 33:108569 (2020). PubMed: 33378676
  • Ghosh A  et al. SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy. Sci Adv 5:eaax9778 (2019). PubMed: 31723605
  • Rehman I  et al. PRMT5-mediated arginine methylation of TDP1 for the repair of topoisomerase I covalent complexes. Nucleic Acids Res 46:5601-5617 (2018). PubMed: 29718323
  • Meisenberg C  et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res 45:1159-1176 (2017). WB ; Human . PubMed: 28180300
View all Publications for this product

客户评价及客户问答

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1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-TDP1 antibody

Inconclusive
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 25 2017

Immunoprecipitation abreview for Anti-TDP1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (Human primary fibroblasts)
Specification
Human primary fibroblasts
Total protein in input
30 µg
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 19 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-TDP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 24 2014

Western blot abreview for Anti-TDP1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient Tris-Gly gel)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 19 2014

Western blot abreview for Anti-TDP1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient Tris-Gly gel)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Blocking step
LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 19 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TDP1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (testis)
Specification
testis
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Frédéric Leduc

Verified customer

提交于 Jan 27 2009

Question

Thank you very much for your e-mail. I am not asking you for a free sample for testing, instead on my own risk I am asking you to sell me some Tdp1 Abs before you advertise them for sale. I am going to use them for my research project as a Western blot probe for this protein in human cell extracts. I will appreciate your help very much.

Read More

Abcam community

Verified customer

Asked on Feb 11 2004

Answer

I was just passed your email about the TDP1 antibody ab4166. I am not sure what you were told before, but we have just published the antibody on the website this week (www.abcam.com/ab4166). So you can go ahead and buy it now. Our collaborator whom we made the antibody with was very happy with how it worked! On another matter, do you know that we are looking for ideas for new antibodies to make in DNA repair? If you have any ideas for antibodies that you think would be useful to the community then please let me know..... If it was something particularly exciting that you were asking for, then we could give you exclusive access to it for 3 months before we started selling it to other researchers. So sorry for the confusion and I hope you have success with the antibody.

Read More

Abcam Scientific Support

回复于 Feb 11 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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