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Anti-TDO2/TDO抗体[OTI2A4] - BSA and Azide free (ab273654)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
  • Western blot - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

Key features and details

  • Mouse monoclonal [OTI2A4] to TDO2/TDO - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-TDO2/TDO抗体[OTI2A4] - BSA and Azide free
    参阅全部 TDO2/TDO 一抗

  • 描述

    小鼠单克隆抗体[OTI2A4] to TDO2/TDO - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein corresponding to Human TDO2/TDO aa 1-450.
    Database link: P48775

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: Transfected HEK-293T cell lysate; IHC-P: Human breast, colon, liver, lung carcinoma, ovary adenocarcinoma, pancreas, endometrium adenocarcinoma, prostate and tonsil tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273654于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000. Predicted molecular weight: 48 kDa.
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/2000. Predicted molecular weight: 48 kDa.
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin.

  • 通路

    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.

  • 序列相似性

    Belongs to the tryptophan 2,3-dioxygenase family.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 3-dioxygenase antibody
      • T23O_HUMAN antibody
      • TDO 2 antibody
      • TDO antibody
      • tdo2 antibody
      • TO antibody
      • TPH2 antibody
      • TRPO antibody
      • Tryptamin 2 3 dioxygenase antibody
      • Tryptamin 2 antibody
      • Tryptophan 2 3 dioxygenase antibody
      • Tryptophan 2 antibody
      • Tryptophan oxygenase antibody
      • Tryptophan pyrrolase antibody
      • Tryptophanase antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

      Immunohistochemical analysis of paraffin-embedded human endometrium adenocarcinoma tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Western blot - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Western blot - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      All lanes : Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654) at 1/2000 dilution

      Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : pCMV6-ENTRY TDO2-transfected HEK-293T whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 48 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

      Immunohistochemical analysis of paraffin-embedded human breast tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

      Immunohistochemical analysis of paraffin-embedded human colon tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

      Immunohistochemical analysis of paraffin-embedded human liver tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

      Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

      Immunohistochemical analysis of paraffin-embedded human ovary adenocarcinoma tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

      Immunohistochemical analysis of paraffin-embedded human prostate tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab273654？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273654 被引用在 1 文献中.

    • Ma W  et al. Kynurenine produced by tryptophan 2,3-dioxygenase metabolism promotes glioma progression through an aryl hydrocarbon receptor-dependent signaling pathway. Cell Biol Int 46:1577-1587 (2022). PubMed: 35702760

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