重组Anti-TCTP抗体[EPR5540] (ab133568)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5540] to TCTP
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TCTP抗体[EPR5540]
参阅全部 TCTP 一抗 -
描述
兔单克隆抗体[EPR5540] to TCTP -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- A431 cells; Raji, HeLa, Jurkat and A431 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5540 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133568于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (3) |
1/10000 - 1/50000. Predicted molecular weight: 20 kDa.
|
ICC/IF |
1/250 - 1/500.
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 20 kDa. |
ICC/IF
1/250 - 1/500. |
靶标
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功能
Involved in calcium binding and microtubule stabilization. -
组织特异性
Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate. -
序列相似性
Belongs to the TCTP family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 7178 Human
- Entrez Gene: 22070 Mouse
- Entrez Gene: 116646 Rat
- Omim: 600763 Human
- SwissProt: P13693 Human
- SwissProt: P63028 Mouse
- SwissProt: P63029 Rat
- Unigene: 374596 Human
see all -
别名
- FLJ27337 antibody
- Fortilin antibody
- Histamine releasing factor antibody
see all
图片
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All lanes : Anti-TCTP antibody [EPR5540] (ab133568) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa -
Overlay histogram showing A431 cells stained with ab133568 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133568, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Immunofluorescence analysis of A431 cells staining TCTP (green) using ab133568 at a 1/250 dilution. Cell nuclei are shown in blue.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab133568 被引用在 10 文献中.
- Chen L et al. Deubiquitylase OTUD3 Mediates Endoplasmic Reticulum Stress through Regulating Fortilin Stability to Restrain Dopaminergic Neurons Apoptosis. Antioxidants (Basel) 12:N/A (2023). PubMed: 37107185
- Pinkaew D et al. Fortilin interacts with TGF-ß1 and prevents TGF-ß receptor activation. Commun Biol 5:157 (2022). PubMed: 35197550
- Zhu X et al. Upregulation of Translationally Controlled Tumor Protein Is Associated With Cervical Cancer Progression. Front Mol Biosci 8:686718 (2021). PubMed: 34589516
- D'Amico S et al. DHA Affects Microtubule Dynamics Through Reduction of Phospho-TCTP Levels and Enhances the Antiproliferative Effect of T-DM1 in Trastuzumab-Resistant HER2-Positive Breast Cancer Cell Lines. Cells 9:N/A (2020). PubMed: 32438775
- Neuhäuser K et al. Assessment of the role of translationally controlled tumor protein 1 (TPT1/TCTP) in breast cancer susceptibility and ATM signaling. Clin Transl Radiat Oncol 15:99-107 (2019). PubMed: 30815593
- Sun R et al. TCTP promotes epithelial-mesenchymal transition in lung adenocarcinoma. Onco Targets Ther 12:1641-1653 (2019). PubMed: 30881019
- Sun Y et al. miR-455-3p functions as a tumor suppressor in colorectal cancer and inhibits cell proliferation by targeting TPT1. Int J Clin Exp Pathol 11:2522-2529 (2018). PubMed: 31938365
- Pinkaew D et al. Fortilin binds IRE1a and prevents ER stress from signaling apoptotic cell death. Nat Commun 8:18 (2017). WB . PubMed: 28550308
- Sawhney S et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . PubMed: 25643152
- Chen K et al. Long-term artificial selection reveals a role of TCTP in autophagy in Mammalian cells. Mol Biol Evol 31:2194-211 (2014). PubMed: 24890374