Anti-TCP1 theta抗体(ab176691)
Key features and details
- Rabbit polyclonal to TCP1 theta
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-TCP1 theta抗体
参阅全部 TCP1 theta 一抗 -
描述
兔多克隆抗体to TCP1 theta -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chicken, Cow, Cynomolgus monkey, Orangutan
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免疫原
Synthetic peptide within Human TCP1 theta aa 498-548. The exact sequence is proprietary.
Sequence:LDTYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQN D
Database link: P50990 -
阳性对照
- HeLa, 293T, Jurkat or Mouse NIH3T3 lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH: 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176691于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 60 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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相关性
TCP1 theta is the theta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double ring. TCP1 theta is a molecular chaperone that assists the folding of proteins upon ATP hydrolysis. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein. -
细胞定位
Cytoplasm. Cytoplasm; cytoskeleton; centrosome -
数据库链接
- Entrez Gene: 418486 Chicken
- Entrez Gene: 281047 Cow
- Entrez Gene: 10694 Human
- Entrez Gene: 12469 Mouse
- SwissProt: Q6EE31 Chicken
- SwissProt: Q3ZCI9 Cow
- SwissProt: P50990 Human
- SwissProt: P42932 Mouse
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别名
- C21orf112 antibody
- CCT 8 antibody
- CCT theta antibody
see all
图片
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Immunoprecipitation - Anti-TCP1 theta antibody (ab176691)
Detection of Human TCP1 theta by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab176691 used for IP at 6 µg/mg lysate. For blotting immunoprecipitated TCP1 theta, ab176691 was used at 0.04 µg/ml.
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Western blot - Anti-TCP1 theta antibody (ab176691)All lanes : Anti-TCP1 theta antibody (ab176691) at 0.04 µg/ml
Lane 1 : HeLa lysate at 50 µg
Lane 2 : HeLa lysate at 15 µg
Lane 3 : 293T lysate at 50 µg
Lane 4 : Jurkat lysate at 50 µg
Lane 5 : Mouse NIH3T3 lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 10 seconds
数据表及文件
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Datasheet download
文献 (0)
ab176691 尚未被引用在任何文献中。
