AB249544

重组Anti-TCP1 eta抗体[EPR10945(B)] - BSA and Azide free

Anti-TCP1 eta antibody [EPR10945(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TCP1 eta antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

查看别名

CCTH, NIP7-1, CCT7, T-complex protein 1 subunit eta, TCP-1-eta, CCT-eta, Chaperonin containing T-complex polypeptide 1 subunit 7, HIV-1 Nef-interacting protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10945(B)] - BSA and Azide free (AB249544)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10945(B)] - BSA and Azide free (AB249544)

This data was developed using ab170861, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling TCP1 eta with ab170861 at a 1/100 dilution (green) DAPI nuclear staining (blue).

Supplier Data

Western blot - Anti-TCP1 eta antibody [EPR10945(B)] - BSA and Azide free (AB249544)

This data was developed using ab170861, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (<a href='/products/primary-antibodies/tcp1-eta-antibody-epr10945b-ab170861'>ab170861</a>) at 1/10000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 59 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-TCP1 eta antibody [EPR10945(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10945(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab249544 is the carrier-free version of ab170861.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The TCP1 eta protein alternatively known as CCT7 is a molecular chaperone involved in protein folding. It is a component of the eukaryotic chaperonin complex known as the T-complex protein 1 (TCP1) ring complex (TRiC). The protein has an approximate mass of 58 kDa and is widely expressed across various tissues. TCP1 eta forms a multi-subunit structure in cells where it assists in the proper folding of newly synthesized polypeptides particularly actin and tubulin.

Biological function summary

TCP1 eta functions as part of the chaperonin-containing TCP1 (CCT) complex. The CCT complex plays a critical role in maintaining cellular proteostasis by promoting the correct folding and assembly of proteins. Ths complex is key for regulatory processes in the cytoskeleton and cell cycle. The folding activity of TCP1 eta is essential for cytoskeletal dynamics given its involvement with structural proteins like actin.

Pathways

TCP1 eta engages significantly in the actin and tubulin folding pathways. These pathways are vital for the formation of the cytoskeleton and spindle apparatus which are necessary for cell shape and division. TCP1 eta interacts with other chaperonin proteins such as TCP1 epsilon within these pathways to ensure efficient folding and stability of target proteins.

TCP1 eta has been linked to certain neurodegenerative diseases and cancer. Misfolded proteins are often associated with conditions like Huntington's disease where impaired CCT function disrupts protein homeostasis. Additionally there are connections between TCP1 eta and the stability of oncogenic proteins implicating its role in cancer progression. Interactions with other proteins such as p53 are relevant for understanding its role in these disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed : 25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). The TRiC complex plays a role in the folding of actin and tubulin (Probable).

See full target information CCT7

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重组Anti-TCP1 eta抗体[EPR10945(B)] - BSA and Azide free