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重组RabMAb

重组Anti-TCP1 epsilon/CCT5抗体[EPR7562] (ab129016)

Reviews (1)Q&A (1)References (7)
Western blot - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
  • OI-RD Scanning - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
  • Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7562] to TCP1 epsilon/CCT5
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TCP1 epsilon/CCT5抗体[EPR7562]
    参阅全部 TCP1 epsilon/CCT5 一抗

  • 描述

    兔单克隆抗体[EPR7562] to TCP1 epsilon/CCT5

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human TCP1 epsilon/CCT5 aa 1-100. The exact sequence is proprietary.

  • 阳性对照

    • HeLa, MOLT4 and A431 cell lysates; Human breast and colon tissues.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab129016于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
WB
1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

  • 疾病相关

    Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive

  • 序列相似性

    Belongs to the TCP-1 chaperonin family.

  • 细胞定位

    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CCT 5 antibody
    • CCT epsilon antibody
    • CCT-epsilon antibody
    • CCT5 antibody
    • CCTE antibody
    • CCTepsilon antibody
    • Chaperonin containing T-complex polypeptide 1, subunit 5 antibody
    • Chaperonin containing TCP 1 epsilon antibody
    • Chaperonin containing TCP 1 subunit 5 antibody
    • Chaperonin containing TCP 1 subunit 5 epsilon antibody
    • Chaperonin containing TCP1 epsilon antibody
    • Chaperonin containing TCP1 subunit 5 antibody
    • Chaperonin containing TCP1 subunit 5 epsilon antibody
    • KIAA0098 antibody
    • T complex protein 1 epsilon subunit antibody
    • T complex protein 1 subunit epsilon antibody
    • T-complex protein 1 subunit epsilon antibody
    • TCP 1 epsilon antibody
    • TCP-1-epsilon antibody
    • TCP1epsilon antibody
    • TCPE antibody
    • TCPE_HUMAN antibody
    see all

图片

  • Western blot - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
    Western blot - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
    All lanes : Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MOLT4 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)

    ab129016, at 1/100 dilution, staining TCP1 epsilon/CCT5 in paraffin-embedded Human breast tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)

    ab129016, at 1/100 dilution, staining TCP1 epsilon/CCT5 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
    OI-RD Scanning - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)
    Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016)

文献 (7)

发表研究结果有使用 ab129016？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab129016 被引用在 7 文献中.

  • Iwasaki M  et al. Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. iScience 25:104289 (2022). PubMed: 35573189
  • Cox A  et al. Chaperonin containing TCP-1 (CCT/TRiC) is a novel therapeutic and diagnostic target for neuroblastoma. Front Oncol 12:975088 (2022). PubMed: 36185250
  • Gestaut D  et al. Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT. Cell 185:4770-4787.e20 (2022). PubMed: 36493755
  • Collier MP  et al. Native mass spectrometry analyses of chaperonin complex TRiC/CCT reveal subunit N-terminal processing and re-association patterns. Sci Rep 11:13084 (2021). PubMed: 34158536
  • Showalter AE  et al. Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis. Sci Rep 10:798 (2020). PubMed: 31964905
  • Hannich JT  et al. 1-Deoxydihydroceramide causes anoxic death by impairing chaperonin-mediated protein folding. Nat Metab 1:996-1008 (2019). PubMed: 32694842
  • Knowlton JJ  et al. The TRiC chaperonin controls reovirus replication through outer-capsid folding. Nat Microbiol 3:481-493 (2018). PubMed: 29531365

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-TCP1 epsilon/CCT5 antibody [EPR7562]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary rat cortical neuron)
Gel Running Conditions
Reduced Denaturing (10-12% SDS-PAGE)
Loading amount
200000 cells
Treatment
Lentivirus mediated overexpression of Avi tagged CCT5
Specification
primary rat cortical neuron
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 27 2020

Question

Customer kindly contacted us regarding use of this product in IP. Theyplan to use this in IP on HEK cells and were hoping we could share some of the details of the IP testing done for this product. What type of cells were used for IP? What concentration of sample was used?

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Answer

The only cell line we tested in IP is HeLa; however, we would recommend using 200-300ul cell lysate per experiment as described below: Immunoprecipitation Protocol 3.1. Mix 200-300 μl of non-denatured cell lysate with appropriate dilutions of primary antibody. 3.2. Incubate overnight at 4 °C with gentle rocking. 3.3. Add 25 μl of Protein G beads (50% bead slurry) and incubate 2 hours at 4 °C with gentle rocking. 3.4. Microcentrifuge for 30 seconds and discard the supernatant. Wash pellet 3 times with cold PBS. 3.5. Resuspend the pellet with 20 μl of 2x SDS reducing sample buffer containing β-mercaptoethanol (5%, v/v) and heat the sample at 95-100 °C for 3 minutes. 3.6. Briefly centrifuge the sample. 3.7. Load 16 μl of sample on to a SDS-PAGE gel or freeze the sample for later use (reboiled for 3 minutes prior loading onto the SDS-PAGE gel). 3.8. Perform Western blotting analysis using HRP-conjugated anti-rabbit IgG as secondary antibody. Recommended Western Blot Protocol.

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