Anti-TCP1 epsilon/CCT5抗体(ab225876)
Key features and details
- Rabbit polyclonal to TCP1 epsilon/CCT5
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-TCP1 epsilon/CCT5抗体
参阅全部 TCP1 epsilon/CCT5 一抗 -
描述
兔多克隆抗体to TCP1 epsilon/CCT5 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Orangutan
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免疫原
Synthetic peptide within Human TCP1 epsilon/CCT5 aa 100-150. The exact sequence is proprietary. (NP_036205.1).
Database link: P48643 -
阳性对照
- IHC-P: Human prostate carcinoma tissue; Mouse teratoma tissue. WB: HEK-293T, Jurkat, HeLa and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab225876 was affinity purified using an epitope specific to TCP1 epsilon/CCT5 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 60 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. -
疾病相关
Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive -
序列相似性
Belongs to the TCP-1 chaperonin family. -
细胞定位
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 22948 Human
- Entrez Gene: 12465 Mouse
- Entrez Gene: 100451325 Orangutan
- Entrez Gene: 294864 Rat
- Omim: 610150 Human
- SwissProt: P48643 Human
- SwissProt: P80316 Mouse
- SwissProt: Q68FQ0 Rat
see all -
别名
- CCT 5 antibody
- CCT epsilon antibody
- CCT-epsilon antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab225876)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).
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Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab225876)All lanes : Anti-TCP1 epsilon/CCT5 antibody (ab225876) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 3 seconds -
Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (ab225876)TCP1 epsilon/CCT5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225876 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225876 at 0.4 µg/ml.
Lane 1: ab225876 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab225876)Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225876 尚未被引用在任何文献中。