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重组RabMAb

重组Anti-TCP1 delta抗体[EPR8495(B)] (ab129072)

Reviews (1) Submit a question References (3)
Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
  • Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8495(B)] to TCP1 delta
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TCP1 delta抗体[EPR8495(B)]
    参阅全部 TCP1 delta 一抗

  • 描述

    兔单克隆抗体[EPR8495(B)] to TCP1 delta

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human TCP1 delta aa 450-550. The exact sequence is proprietary.

  • 阳性对照

    • HeLa, Jurkat, Ramos and HepG2 whole cell lysate (ab7900); Human stomach tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR8495(B)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab129072于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.

靶标

  • 相关性

    The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back to back rings, each containing 8 unique but homologous subunits (e.g. CCT3). CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin. TCP1 delta belongs to the TCP1 chaperonin family, and is a molecular chaperone; assisting the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin.

  • 细胞定位

    Cytoplasmic > Cytoskeleton

  • 数据库链接

    • 别名

      • CCT delta antibody
      • CCT4 antibody
      • CCTD antibody
      • chaperonin containing t-complex polypeptide 1, delta subunit antibody
      • chaperonin containing TCP1, subunit 4 (delta) antibody
      • SRB antibody
      • Stimulator of TAR RNA binding antibody
      • T complex protein 1 subunit delta antibody
      • TCP-1-delta antibody
      see all

    图片

    • Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
      Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
      All lanes : Anti-TCP1 delta antibody [EPR8495(B)] (ab129072) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 58 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)

      ab129072, at 1/50 dilution, staining TCP1 delta in paraffin-embedded Human stomach tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)
      Anti-TCP1 delta antibody [EPR8495(B)] (ab129072)

    文献 (3)

    发表研究结果有使用 ab129072？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab129072 被引用在 3 文献中.

    • Wang G  et al. Anticarin-β shows a promising anti-osteosarcoma effect by specifically inhibiting CCT4 to impair proteostasis. Acta Pharm Sin B 12:2268-2279 (2022). PubMed: 35646538
    • Cox A  et al. Chaperonin containing TCP-1 (CCT/TRiC) is a novel therapeutic and diagnostic target for neuroblastoma. Front Oncol 12:975088 (2022). PubMed: 36185250
    • Showalter AE  et al. Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis. Sci Rep 10:798 (2020). PubMed: 31964905

    客户评价及客户问答

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    Western blot abreview for Anti-TCP1 delta antibody [EPR8495(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (primary embryonic rat cortical neuron)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    200000 cells
    Treatment
    Lentivirus mediated knockdown of CCT2 induced down regulation of CCT4 in primary rat cortical neurons
    Specification
    primary embryonic rat cortical neuron
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 26 2020

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