AB129072

重组Anti-TCP1 delta抗体[EPR8495(B)]

Name: TCP1 delta抗体[EPR8495(B)] (ab129072)| Abcam中文官网
Brand: Abcam Limited
SKU: AB129072
Price: 2385 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-TCP1 delta antibody [EPR8495(B)]

RabMAb
Recombinant
了解详情

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal TCP1 delta antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

查看别名

CCTD, SRB, CCT4, T-complex protein 1 subunit delta, TCP-1-delta, CCT-delta, Chaperonin containing T-complex polypeptide 1 subunit 4, Stimulator of TAR RNA-binding

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (AB129072)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (AB129072)

ab129072 at 1/50 dilution staining TCP1 delta in paraffin-embedded Human stomach tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (AB129072)

All lanes:

Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Ramos cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 60 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-TCP1 delta antibody [EPR8495(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8495(B)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TCP1 delta also known as CCT4 is a subunit of the chaperonin containing TCP1 complex. This protein has a molecular mass of approximately 60 kDa. It is part of a group of proteins in eukaryotic cells known as group II chaperonins. TCP1 delta is expressed in various tissues with higher levels found in brain testis and ovary. It plays a role in protein folding which is important for proper cellular functions.

Biological function summary

TCP1 delta functions as part of the chaperonin complex assisting in the folding of cytoskeletal proteins such as actin and tubulin. This complex is critical for maintaining cellular integrity and function by ensuring proper protein configuration. TCP1 delta influences cell cycle regulation and proliferation through its role in folding key components of the cytoskeleton. Formation of this complex occurs in the cytoplasm where it effectively brings together other subunits to undertake its biological roles.

Pathways

TCP1 delta interacts significantly in the protein folding and cytoskeleton organization pathways. It collaborates with other proteins like CCT1 and CCT5 within the TCP-1 ring complex ensuring accurate protein assembly. The protein folding pathway where TCP1 delta operates is essential for cell cycle progression and maintaining cellular homeostasis while also playing a role in the signal transduction involving the cytoskeleton.

TCP1 delta connections appear in some neurodegenerative diseases and cancer. Abnormal function or expression of TCP1 delta relates to misfolding and aggregation of proteins a hallmark of conditions like Huntington’s disease. Moreover dysregulation of TCP1 delta activity is observed in certain cancers where it might interact with oncogenic proteins affecting cell growth and division. Understanding TCP1 delta's role in these diseases helps in identifying potential therapeutic targets.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638).

See full target information CCT4

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107843 PubMed39357829

2024

N-methyladenosine modification of SLC38A7 promotes cell migration, invasion, oxidative phosphorylation, and mitochondrial function in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Hua,Wei-Jun Hua,Cun-Cheng Feng,Qiu-Wei Zhu

Frontiers in oncology 12:975088 PubMed36185250

2022

Chaperonin containing TCP-1 (CCT/TRiC) is a novel therapeutic and diagnostic target for neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Cox,Daniel Nierenberg,Oscar Camargo,Eunkyung Lee,Amr S Khaled,Joseph Mazar,Rebecca J Boohaker,Tamarah J Westmoreland,Annette R Khaled

Acta pharmaceutica Sinica. B 12:2268-2279 PubMed35646538

2022

Anticarin- shows a promising anti-osteosarcoma effect by specifically inhibiting CCT4 to impair proteostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Gan Wang,Min Zhang,Ping Meng,Chengbo Long,Xiaodong Luo,Xingwei Yang,Yunfei Wang,Zhiye Zhang,James Mwangi,Peter Muiruri Kamau,Zhi Dai,Zunfu Ke,Yi Zhang,Wenlin Chen,Xudong Zhao,Fei Ge,Qiumin Lv,Mingqiang Rong,Dongsheng Li,Yang Jin,Xia Sheng,Ren Lai

Scientific reports 10:798 PubMed31964905

2020

Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anne E Showalter,Ana C Martini,Daniel Nierenberg,Kristen Hosang,Naima Ahmed Fahmi,Priya Gopalan,Amr S Khaled,Wei Zhang,Annette R Khaled

View all publications

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重组Anti-TCP1 delta抗体[EPR8495(B)]