重组Anti-TCP1 alpha/CCTA抗体[EPR4082] (ab109126)
![Western blot - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)](https://www.abcam.cn/ps/products/109/ab109126/Images/ab109126-121722-anti-tcp1-alpha-antibody-epr4082-western-blot.jpg "Western blot - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TCP1 alpha/CCTA抗体[EPR4082]
参阅全部 TCP1 alpha/CCTA 一抗 -
描述
兔单克隆抗体[EPR4082] to TCP1 alpha/CCTA -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, ECV-304, MCF7 and Daudi cell lysates; Human testis tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4082 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109126于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
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| IP |
1/10 - 1/100.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
|
IP
1/10 - 1/100. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. -
序列相似性
Belongs to the TCP-1 chaperonin family. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 6950 Human
- Entrez Gene: 21454 Mouse
- Entrez Gene: 100302435 Rabbit
- Entrez Gene: 24818 Rat
- Omim: 186980 Human
- SwissProt: P17987 Human
- SwissProt: P11983 Mouse
- SwissProt: P28480 Rat
see all -
别名
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
see all
图片
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Western blot - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : ECV-304 cell lysates
Lane 3 : MCF7 cell lysates
Lane 4 : Daudi cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa -
![Immunoprecipitation - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)](https://www.abcam.cn/ps/products/109/ab109126/Images/ab109126-294607-anti-tcp1-alpha-antibody-epr4082-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha/CCTA in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate.
Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate
For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126) at 1/1000 dilution
Lane 1 : ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2 : PBS instead of ab109126 in ECV-304 whole cell lysate
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)](https://www.abcam.cn/ps/products/109/ab109126/Images/ab109126-121721-anti-tcp1-alpha-antibody-epr4082-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)ab109126, at 1/100 dilution staining TCP1 in paraffin-embedded Human testis tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)](https://www.abcam.cn/ps/products/109/ab109126/Images/ab109126-4-benefits-of-recombinant-antibodies.png)
Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab109126 被引用在 5 文献中.
- Llamas E et al. The intrinsic chaperone network of Arabidopsis stem cells confers protection against proteotoxic stress. Aging Cell 20:e13446 (2021). PubMed: 34327811
- Zhong XL et al. Low expression of TCP1 (T-Complex 1) and PSMC1 (Proteasome 26S subunit, ATPase 1) in heterotopic ossification during ankylosing spondylitis. Bioengineered 12:7459-7469 (2021). PubMed: 34612770
- Chen X et al. TCP1 increases drug resistance in acute myeloid leukemia by suppressing autophagy via activating AKT/mTOR signaling. Cell Death Dis 12:1058 (2021). PubMed: 34750375
- Intawicha P et al. Proteomic profiling of rabbit embryonic stem cells derived from parthenotes and fertilized embryos. PLoS One 8:e67772 (2013). WB, ICC/IF ; Rabbit . PubMed: 23861804
- Gao X et al. Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins. J Biol Chem 288:25760-8 (2013). PubMed: 23888055