JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB65212

Anti-TCOF1抗体

Anti-TCOF1 antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Polyclonal TCOF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TCOF1.

查看别名

Treacle protein, Treacher Collins syndrome protein, TCOF1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (AB65212)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (AB65212)

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab65212 at a 1 : 50 dilution.
Left image untreated.
Right image treated with immunising peptide.

Western blot - Anti-TCOF1 antibody (AB65212)
  • WB

Unknown

Western blot - Anti-TCOF1 antibody (AB65212)

The amount of positive control loading for the WB is 5-30 ug of total protein.
The amount of the peptide for the WB is 5-10 ug.

All lanes:

Western blot - Anti-TCOF1 antibody (ab65212) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells

Lane 2:

Extracts from Jurkat cells, plus immunising peptide

Predicted band size: 152 kDa

Observed band size: 152 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human TCOF1. The exact immunogen used to generate this antibody is proprietary information.

Q13428

style
left-column

替代产品

Primary Antibodies

AB224544

Anti-TCOF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (AB224544)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB244287

Anti-UBF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody (AB244287)

  • 5
  • 2
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB22758

Anti-Nucleolin antibody

BOND RX™ Validated

Western blot - Anti-Nucleolin antibody (AB22758)

  • 4
  • 8
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TCOF1 also known as Treacle protein possesses a mechanical role in the nucleolus involved in ribosomal DNA transcription. This protein has a mass of approximately 153 kDa. It is expressed in a wide range of tissues but shows significant activity in neural crest cells during early development. TCOF1 helps in maintaining the integrity of ribosomal DNA and regulates the production of ribosomal RNA. Correct regulation of ribosomal RNA is essential for cellular function and survival.
Biological function summary

TCOF1 is critical in craniofacial development. The protein works as a part of the treacle complex interacting with RNA polymerase I and upstream binding factor 1 (UBF1) to facilitate ribosomal RNA synthesis. By supporting the transcriptional machinery TCOF1 impacts cellular proliferation and growth particularly in tissues derived from neural crest cells. This regulation of ribosomal RNA transcription links to various aspects of cellular and developmental biology.

Pathways

TCOF1 plays an important role in the ribosome biogenesis and p53 signaling pathways. In ribosome biogenesis TCOF1 interacts with proteins like NOLC1 to influence RNA polymerase I activity. The p53 signaling pathway involves TCOF1 as a ribosome biogenesis dysfunction can activate p53 which is an important player in the cellular checkpoint and apoptosis. This position in pathways hints at TCOF1's involvement in both regular growth processes and stress response mechanisms in cells.

TCOF1 mutations lead to Treacher Collins syndrome a condition resulting in craniofacial deformities. The disorder involves impaired development of facial bones due to disrupted ribosomal RNA transcription. Protein partners include POLR1C and POLR1D which also associate with Treacher Collins syndrome when mutated. TCOF1's role in RNA synthesis highlights its relevance in these developmental anomalies and provides insight into potential therapeutic targets.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed : 12777385, PubMed : 26399832). Required for neural crest specification : following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed : 26399832).
See full target information TCOF1
style
left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 19:e1010854 PubMed37639467

2023

rRNA transcription is integral to phase separation and maintenance of nucleolar structure.

Applications

Unspecified application

Species

Unspecified reactive species

Soma Dash,Maureen C Lamb,Jeffrey J Lange,Mary C McKinney,Dai Tsuchiya,Fengli Guo,Xia Zhao,Timothy J Corbin,MaryEllen Kirkman,Kym Delventhal,Emma L Moore,Sean McKinney,Rita Shiang,Paul A Trainor

Molecular genetics & genomic medicine 11:e2150 PubMed36808708

2023

Genetic mutations in ribosomal biogenesis gene TCOF1 identified in human neural tube defects.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wang,Haiqin Cheng,Qin Zhang,Jin Guo

The journal of histochemistry and cytochemistry : 64:669-686 PubMed27680669

2016

Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Franek,Alena Kovaříková,Eva Bártová,Stanislav Kozubek

Epigenetics & chromatin 7:39 PubMed25587355

2014

HP1β-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs.

Applications

ICC/IF

Species

Mouse

Lenka Stixová,Petra Sehnalová,Soňa Legartová,Jana Suchánková,Tereza Hrušková,Stanislav Kozubek,Dmitry V Sorokin,Pavel Matula,Ivan Raška,Aleš Kovařík,Jaroslav Fulneček,Eva Bártová

Human molecular genetics 19:3491-501 PubMed20591827

2010

CHD7 functions in the nucleolus as a positive regulator of ribosomal RNA biogenesis.

Applications

WB, ChIP

Species

Mouse, Mouse

Gabriel E Zentner,Elizabeth A Hurd,Michael P Schnetz,Lusy Handoko,Chuanping Wang,Zhenghe Wang,Chialin Wei,Paul J Tesar,Maria Hatzoglou,Donna M Martin,Peter C Scacheri
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com