Anti-TCF-25抗体(ab225627)
Key features and details
- Rabbit polyclonal to TCF-25
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-TCF-25抗体
参阅全部 TCF-25 一抗 -
描述
兔多克隆抗体to TCF-25 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Xenopus tropicalis
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免疫原
Synthetic peptide within Human TCF-25 aa 1-50. The exact sequence is proprietary. (NP_055787.1).
Database link: Q9BQ70 -
阳性对照
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab225627 was affinity purified using an epitope specific to TCF-25 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225627于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
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| IP |
Use a concentration of 2 - 5 µg/ml.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa. |
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IP
Use a concentration of 2 - 5 µg/ml. |
靶标
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功能
May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development. -
组织特异性
In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart. -
序列相似性
Belongs to the TCF25 family. -
结构域
The C-terminal region mediates transcriptional repression. -
细胞定位
Nucleus. Some staining in the cytosol. - Information by UniProt
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数据库链接
- Entrez Gene: 100760643 Chinese hamster
- Entrez Gene: 618386 Cow
- Entrez Gene: 608053 Dog
- Entrez Gene: 101129770 Gorilla
- Entrez Gene: 22980 Human
- Entrez Gene: 66855 Mouse
- Entrez Gene: 100620736 Pig
- Entrez Gene: 292082 Rat
see all -
别名
- FKSG26 antibody
- hKIAA1049 antibody
- Hulp1 antibody
see all
图片
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Western blot - Anti-TCF-25 antibody (ab225627)All lanes : Anti-TCF-25 antibody (ab225627) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-TCF-25 antibody (ab225627)TCF-25 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225627 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225627 at 0.4 µg/ml.
Lane 1: ab225627 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225627 尚未被引用在任何文献中。