AB225627

Anti-TCF-25抗体

Name: TCF-25抗体 (ab225627)| Abcam中文官网
Brand: Abcam Limited
SKU: AB225627
Price: 4748 CNY
Availability: InStock

Anti-TCF-25 antibody

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(1 Publication)

Rabbit Polyclonal TCF-25 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TCF25 aa 1-50.

查看别名

KIAA1049, NULP1, FKSG26, TCF25, Ribosome quality control complex subunit TCF25, Nuclear localized protein 1, Transcription factor 25, TCF-25

2 Images

Supplier Data

Immunoprecipitation - Anti-TCF-25 antibody (AB225627)

TCF-25 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225627 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225627 at 0.4 μg/ml.

Lane 1 : ab225627 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TCF-25 antibody (ab225627)

Predicted band size: 77 kDa

false

Supplier Data

Western blot - Anti-TCF-25 antibody (AB225627)

All lanes:

Western blot - Anti-TCF-25 antibody (ab225627) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 77 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human TCF25 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BQ70

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab225627 was affinity purified using an epitope specific to TCF-25 immobilized on solid support.

存储溶液

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TCF-25 also known as Transcription Factor 25 plays an important role in gene expression by regulating transcription processes. Its protein mass is approximately 25 kDa. TCF-25 is expressed broadly across several tissues with particular prominence in the brain and heart. This expression pattern suggests its involvement in critical cellular processes in these organ systems.

Biological function summary

TCF-25 modulates expression of genes involved in development and differentiation. It functions as a part of a complex with other transcription factors to regulate target genes. This complex interaction enhances its ability to control biological processes maintaining cellular homeostasis and supporting the progression of various developmental stages.

Pathways

Studies show TCF-25 plays an influential role in both Wnt and Notch signaling pathways. These pathways are important for cell fate determination and tissue development. Within these pathways TCF-25 interacts with proteins such as beta-catenin in the Wnt signaling pathway and works alongside Notch receptors in the Notch signaling pathway illustrating its integration into these significant cellular signaling networks.

Research finds TCF-25 linked to cardiovascular diseases and neuronal development disorders. Abnormalities in TCF-25 expression or function contribute to pathogenesis in conditions like congenital heart defects where its partnership with proteins like GATA4 and NKX2-5 is essential. Additionally misregulation of TCF-25 can impact neural processes potentially leading to neurological disorders through its interactions with other transcription factors.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation (PubMed : 30244831). In the RQC complex, required to promote formation of 'Lys-48'-linked polyubiquitin chains during ubiquitination of incompletely synthesized proteins by LTN1 (PubMed : 30244831). Also acts as a transcriptional repressor : represses transcription of SRF in vitro and so may play a role in heart development (PubMed : 16574069). May play a role in cell death control (By similarity).

See full target information TCF25

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer gene therapy 30:1007-1017 PubMed36973424

2023

E2F1-mediated KDM4A-AS1 up-regulation promotes EMT of hepatocellular carcinoma cells by recruiting ILF3 to stabilize AURKA mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Ming Shen,Di Zhang,Ping Xiao,Bin Qu,Yi-Fan Sun

View all publications

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Anti-TCF-25抗体

Anti-TCF-25 antibody

Immunoprecipitation - Anti-TCF-25 antibody (AB225627)

Western blot - Anti-TCF-25 antibody (AB225627)