重组Anti-TC10抗体[Y304] (ab32079)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y304] to TC10
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TC10抗体[Y304]
参阅全部 TC10 一抗 -
描述
兔单克隆抗体[Y304] to TC10 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293 cell lysate, HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y304 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32079于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
|
ICC/IF |
1/250 - 1/500.
|
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
ICC/IF
1/250 - 1/500. |
靶标
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功能
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia. -
序列相似性
Belongs to the small GTPase superfamily. Rho family. -
翻译后修饰
May be post-translationally modified by both palmitoylation and polyisoprenylation. -
细胞定位
Cytoplasm. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 23433 Human
- Entrez Gene: 85428 Rat
- Omim: 605857 Human
- SwissProt: P17081 Human
- SwissProt: Q9JJL4 Rat
- Unigene: 709193 Human
- Unigene: 4169 Rat
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别名
- ARHQ antibody
- Ras homolog gene family member Q antibody
- RAS like family 7 member A antibody
see all
图片
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Anti-TC10 antibody [Y304] (ab32079) at 1/1000 dilution + 293 cell lysate
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of TC10 expression in HeLa cells using 1/250 ab 32079.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab32079 被引用在 6 文献中.
- Koch D et al. Obscurin Rho GEF domains are phosphorylated by MST-family kinases but do not exhibit nucleotide exchange factor activity towards Rho GTPases in vitro. PLoS One 18:e0284453 (2023). PubMed: 37079638
- Liang J et al. Inhibition of the mevalonate pathway enhances cancer cell oncolysis mediated by M1 virus. Nat Commun 9:1524 (2018). PubMed: 29670091
- Fujita A et al. GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. PLoS One 8:e79689 (2013). ICC/IF . PubMed: 24223996
- Heasman SJ et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). WB ; Human . PubMed: 20733052
- Fausther-Bovendo H et al. HIV gp41 engages gC1qR on CD4+ T cells to induce the expression of an NK ligand through the PIP3/H2O2 pathway. PLoS Pathog 6:e1000975 (2010). WB ; Human . PubMed: 20617170
- Yasuda S et al. An essential role of Cdc42-like GTPases in mitosis of HeLa cells. FEBS Lett 580:3375-80 (2006). PubMed: 16716304