Anti-Tbx6抗体(ab38883)
Key features and details
- Rabbit polyclonal to Tbx6
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Tbx6抗体
参阅全部 Tbx6 一抗 -
描述
兔多克隆抗体to Tbx6 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Mouse Tbx6 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab26243) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab38883于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa). |
靶标
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功能
Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation. -
组织特异性
Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus. -
序列相似性
Contains 1 T-box DNA-binding domain. -
发展阶段
Expressed during gastrulation and during a second phase in some adult tissues. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6911 Human
- Entrez Gene: 21389 Mouse
- Omim: 602427 Human
- SwissProt: O95947 Human
- SwissProt: P70327 Mouse
- Unigene: 198301 Human
- Unigene: 727 Mouse
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别名
- DFNB 67 antibody
- DFNB67 antibody
- SCDO5 antibody
see all
图片
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All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Lung (Mouse) Tissue Lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lane 4 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab38883 被引用在 6 文献中.
- Budjan C et al. Paraxial mesoderm organoids model development of human somites. Elife 11:N/A (2022). PubMed: 35088712
- Sanaki-Matsumiya M et al. Periodic formation of epithelial somites from human pluripotent stem cells. Nat Commun 13:2325 (2022). PubMed: 35484123
- Bredenkamp N et al. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency. Stem Cell Reports 13:1083-1098 (2019). PubMed: 31708477
- Liu C et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. Stem Cell Reports 11:43-57 (2018). PubMed: 30008328
- Chal J et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc 11:1833-50 (2016). PubMed: 27583644
- Gouti M et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol 12:e1001937 (2014). ICC/IF ; Mouse . PubMed: 25157815