Anti-Tbx3抗体(ab99302)
Key features and details
- Rabbit polyclonal to Tbx3
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Tbx3抗体
参阅全部 Tbx3 一抗 -
描述
兔多克隆抗体to Tbx3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- This antibody gave a positive signal in nuclear extracts from the following cell lines: HepG2; MCF7. IHC-P (FFPE): Human Placenta (Normal) tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 5 µg/ml.
|
| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
靶标
-
功能
Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. -
组织特异性
Widely expressed. -
疾病相关
Defects in TBX3 are the cause of ulnar-mammary syndrome (UMS) [MIM:181450]. UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands. -
序列相似性
Contains 1 T-box DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 374202 Chicken
- Entrez Gene: 6926 Human
- Entrez Gene: 21386 Mouse
- Entrez Gene: 353305 Rat
- Omim: 601621 Human
- SwissProt: O15119 Human
- SwissProt: P70324 Mouse
- SwissProt: Q7TST9 Rat
see all -
别名
- Bladder cancer related protein XHL antibody
- T box 3 antibody
- T-box protein 3 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 antibody (ab99302)
ab99302 staining Tbx3 in HepG2 cells. The cells were fixed with 4% Paraformaldehyde (10min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab99302 at 5μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) pre-adsorbed, at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) pre-adsorbed, at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Perkin Elmer, Operetta CLS).
-
Western blot - Anti-Tbx3 antibody (ab99302)All lanes : Anti-Tbx3 antibody (ab99302) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 2 :MCF7 nuclear extract lysate (ab14860)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Additional bands at: 22 kDa, 77 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Ab99302 should recognise all three isoforms of Tbx3. We are probably observing Isoform I and Isoform II on this Western Blot image. The molecular weights of Isoform I and Isoform II are 77- and 79-kDa respectively. -
Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 antibody (ab99302)This image is courtesy of an Abreview by Zoe Burke.ab99302 staining Tbx3 in the AR42J-B13 rat pancreatic tumor cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton/PBS and blocked with blocking buffer for 60 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/5000.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (26)
ab99302 被引用在 26 文献中.
- Tsujikawa K et al. Developmentally interdependent stretcher-compressor relationship between the embryonic brain and the surrounding scalp in the preosteogenic head. Dev Dyn 251:1107-1122 (2022). PubMed: 34997665
- Ishibashi R et al. Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci Rep 12:17775 (2022). PubMed: 36272994
- Li H et al. Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing. Cells 12:N/A (2022). PubMed: 36611882
- Damerell V et al. The c-Myc/TBX3 Axis Promotes Cellular Transformation of Sarcoma-Initiating Cells. Front Oncol 11:801691 (2021). PubMed: 35145908
- Peres J et al. TBX3 Promotes Melanoma Migration by Transcriptional Activation of ID1, which Prevents Activation of E-Cadherin by MITF. J Invest Dermatol 141:2250-2260.e2 (2021). PubMed: 33744299